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Invitrogen™ Caspase 3 Polyclonal Antibody, Rabbit IgG, 100 µL
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Specifications:
| Application | Western Blot, Immunohistochemistry, Immunoprecipitation (IP) | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Caspase 3 Polyclonal Antibody is a highly specific antibody designed to detect caspase 3 in a variety of experimental applications, including Western blotting (WB), Immunohistochemistry (IHC), and Immunocytochemistry (ICC/IF). Caspase 3 is a key enzyme involved in the apoptosis (programmed cell death) pathway, and its activation is a crucial marker for detecting apoptosis. This antibody is derived from rabbit IgG and is ideal for protein analysis and cellular apoptosis studies.
The antibody specifically recognizes caspase 3 in human, mouse, and rat species, allowing for cross-species analysis. It has been tested for various applications, ensuring its reliability and consistency across a range of experimental methods.
Key Features:
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Specific to Caspase 3:
- Recognizes caspase 3 (also known as Apopain or CPP32), a key player in apoptosis.
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Applications:
- Tested for use in Western Blot (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin), Immunocytochemistry (ICC/IF), and Immunoprecipitation (IP).
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Species Reactivity:
- Human, Mouse, and Rat.
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Dilution Range:
- Western Blot (WB): 1:500-1:10,000
- Immunohistochemistry (IHC): 1:100-1:1,000
- Immunocytochemistry (ICC/IF): 1:100-1:1,000
- Immunoprecipitation (IP): 1:100-1:500
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High Purity:
- Purified by antigen affinity chromatography to ensure high specificity and minimal non-specific binding.
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Unconjugated:
- This unconjugated antibody allows flexibility for various labeling techniques, depending on your experimental requirements.
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Optimized for Apoptosis Studies:
- Ideal for apoptosis research, where monitoring caspase 3 activation is critical for understanding cell death and disease mechanisms.
Specifications:
| Feature | Details |
|---|---|
| Host/Isotype | Rabbit IgG |
| Application | Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC/IF), Immunoprecipitation (IP) |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Caspase 3 |
| Conjugate | Unconjugated |
| Concentration | 0.15 mg/mL |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
Positive Controls:
- HeLa Cells, PC-12, Jurkat cells, and others, treated with Cisplatin or under apoptotic conditions.
Application Notes:
The Caspase 3 Polyclonal Antibody is essential for studying apoptosis pathways in various cell types, particularly when cells undergo programmed cell death. It is highly recommended in Western blot analysis for detecting cleaved Caspase 3 and in Immunohistochemistry for identifying apoptotic cells in tissue sections.
Related Products:
- Secondary Antibodies
- Isotype Controls
- Loading Controls
- Electrophoresis WB
- Transfer Stacks for WB
Citations & References:
- This antibody has been referenced in Western blot and IHC applications for detecting cleaved caspase 3 in various studies, including those on cancer, neurodegenerative diseases, and drug-induced apoptosis.
The Invitrogen™ Caspase 3 Polyclonal Antibody is a reliable and high-performance tool for apoptosis research, enabling the detection of caspase 3 activation in Western blot, Immunohistochemistry, Immunocytochemistry, and Immunoprecipitation applications. Its specificity, purity, and reliability make it an essential reagent for cell death studies, biomedical research, and disease mechanism exploration.