Invitrogen™ Mouse β-Actin Monoclonal Antibody (15G5A11/E2), 100 µg

The Invitrogen™ Mouse β-Actin Monoclonal Antibody (15G5A11/E2) is a highly specific monoclonal antibody developed to detect β-actin in various biological samples, including human, mouse, and rat tissues. β-actin is a crucial component of the cytoskeleton and is widely expressed in all eukaryotic cells. It plays an essential role in cell motility, structure, and integrity, making it an ideal loading control in Western blotting (WB) and Immunocytochemistry (ICC/IF).

The 15G5A11/E2 clone is designed for use in Western blotting, Immunohistochemistry (IHC), Immunocytochemistry (ICC/IF), and Immunoprecipitation (IP) applications. It provides consistent detection of β-actin across a variety of experimental setups and is highly effective for studying cytoskeletal function, cell signaling, and cellular dynamics.

Key Features:

1. Highly Specific Detection of β-Actin:
   • Specifically recognizes β-actin, a cytoskeletal protein involved in cell motility, structural integrity, and signal transduction.
2. Wide Species Reactivity:
   • This antibody is reactive with human, mouse, and rat samples, enabling cross-species studies and applications.
3. Versatile Applications:
   • Western Blotting (WB): 1-3 µg/mL
   • Immunohistochemistry (IHC): 1:10-1:50
   • Immunocytochemistry (ICC/IF): 1-2 µg/mL
   • Immunoprecipitation (IP): 2 µg
   • Suitable for detecting β-actin in cell lysates, tissue sections, and fixed or live cells.
4. Purification:
   • Purified by Protein A/G affinity chromatography, ensuring high specificity and minimal non-specific binding.
5. Unconjugated:
   • This antibody is unconjugated, offering flexibility for custom labeling and use in a variety of detection systems.
6. Concentration:
   • The antibody is supplied at a concentration of 1 mg/mL for easy use and dilution.
7. Storage and Handling:
   • Store at 4°C short-term, or -20°C long-term. The antibody contains 0.05% sodium azide as a preservative.
8. Clone 15G5A11/E2:
   • This monoclonal antibody comes from clone 15G5A11/E2, ensuring consistent performance and reliable results across experiments.

Specifications:

Applications:

1. Western Blotting (WB):
   • Detect β-actin in cell lysates and protein extracts to ensure equal loading and normalization in protein expression analysis.
2. Immunohistochemistry (IHC):
   • Detect β-actin in tissue sections, providing insight into cell structure and cytoskeletal integrity.
3. Immunocytochemistry (ICC/IF):
   • Visualize β-actin in live or fixed cells under fluorescence microscopy for cellular studies.
4. Immunoprecipitation (IP):
   • Isolate β-actin complexes for studying protein-protein interactions and cell signaling.
5. Cell Signaling Research:
   • Used in signal transduction studies to assess actin dynamics and its role in cell motility and integrity.
6. Cancer and Disease Research:
   • Monitor cytoskeletal changes in cancer cells and diseased tissues to study cell migration, invasion, and proliferation.

Positive Control:

• HeLa cells, PC-12 cells, or Jurkat cells can be used as positive controls for β-actin detection in Western blot and IHC applications.

Target Information:

β-Actin (ACTB) is one of the most abundant proteins in eukaryotic cells, forming part of the cytoskeleton and contributing to cell shape, motility, and intracellular transport. It is expressed in all cell types and is a critical component of cell division, cell signaling, and mechanical support. β-Actin is often used as a loading control in Western blot assays due to its ubiquitous expression and stable levels across various experimental conditions.

The Invitrogen™ Mouse β-Actin Monoclonal Antibody (15G5A11/E2) is an essential tool for cell biology research, providing high specificity and reliable performance in applications such as Western blotting, Immunohistochemistry, Immunocytochemistry, and Immunoprecipitation. With its ability to detect β-actin, this antibody is indispensable for normalization studies and for investigating cytoskeletal dynamics, cell signaling, and apoptotic processes.