Vircell LEPTOSPIRA IgM ELISA Kit

The Vircell Leptospira IgM ELISA Kit is a high-performance indirect immunoenzyme assay designed for the detection of IgM antibodies against Leptospira interrogans in human serum or plasma. This kit is ideal for diagnosing leptospirosis, a bacterial zoonosis caused by Leptospira interrogans, and provides a user-friendly, efficient solution suitable for manual and automated ELISA systems.

Key Features

1. IgM-Specific Detection:
   • Designed to detect IgM antibodies, providing critical information for diagnosing acute leptospirosis infections.
2. Ready-to-Use Reagents:
   • Pre-prepared reagents reduce handling time and minimize variability.
3. Simplified Sample Handling:
   • No pre-dilution required; serum/plasma samples can be directly added to the wells.
4. Automation Compatibility:
   • Suitable for integration into automated ELISA systems, enabling high-throughput testing.
5. Enhanced Workflow Efficiency:
   • Break-apart wells, color-coded plates, and liquid reagents simplify and optimize workflows.
6. Stable Reagents:
   • Lyophilized conjugates (when required) ensure long-term stability and consistent performance.

Principle of the Assay

The Vircell Leptospira IgM ELISA Kit employs an indirect ELISA methodology:

1. Antigen Binding:
   • Antigens specific to Leptospira interrogans are adsorbed onto the surface of polystyrene wells.
2. Sample Interaction:
   • IgM antibodies in the sample bind to the adsorbed antigens. Unbound immunoglobulins are washed off.
3. Conjugate Reaction:
   • An enzyme-labeled anti-human globulin binds to the antigen-antibody complex.
4. Signal Development:
   • TMB substrate is added, producing a blue-colored solution. Upon addition of the stop solution, the color changes to yellow.
5. Quantification:
   • The optical density (OD) is measured at 450 nm, and the presence of IgM antibodies is determined based on OD values.

Applications and Use Cases

1. Clinical Diagnostics:

• Acute Leptospirosis Detection:
  • Identify recent Leptospira interrogans infections by detecting IgM antibodies.
• Serological Screening:
  • Confirm the presence of specific IgM antibodies in suspected leptospirosis cases.

2. Epidemiological Studies:

• Seroprevalence Monitoring:
  • Assess the prevalence of Leptospira exposure in populations.
• Outbreak Investigation:
  • Track and identify sources of leptospirosis outbreaks.

3. Laboratory Research:

• Viral Immunology Studies:
  • Explore the immune response to Leptospira infections.
• Pathogen-Specific Antibody Profiling:
  • Study IgM antibody responses to Leptospira interrogans.

Kit Components

Assay Specifications

Advantages

1. High Sensitivity and Specificity:
   • Accurate detection of Leptospira IgM antibodies with minimal cross-reactivity.
2. Simplified Handling:
   • Pre-dilution-free protocol saves time and reduces variability.
3. Flexible Testing:
   • Break-apart wells allow for customized testing sizes.
4. Consistent Performance:
   • Stable reagents ensure reproducible results across batches.
5. Efficient Workflow:
   • Automation compatibility supports high-throughput testing.

Storage and Stability

• Storage Conditions: Store all components at 2–8°C.
• Shelf Life: Use before the expiration date indicated on the product label.

The Vircell Leptospira IgM ELISA Kit provides a reliable, sensitive, and efficient solution for detecting Leptospira interrogans IgM antibodies in human serum and plasma. Its simplified protocol, ready-to-use reagents, and compatibility with automated systems make it an excellent choice for clinical diagnostics, research, and epidemiological studies.