Sigma-Aldrich NutriSelect® Plus, Middlebrook 7H9 Broth Base

Sigma-Aldrich NutriSelect® Plus Middlebrook 7H9 Broth Base

Middlebrook 7H9 Broth Base is a specialized liquid culture medium used for the isolation, cultivation, and drug susceptibility testing of Mycobacterium species. Its formulation includes essential inorganic compounds, amino acids, and growth factors necessary for the propagation of Mycobacteria, including Mycobacterium tuberculosis and Mycobacterium bovis. When supplemented with Middlebrook ADC Growth Supplement, the medium offers optimal growth conditions for both research and clinical microbiology applications.

Key Features

1. Supports Rapid Growth
   • Liquid medium promotes faster growth of Mycobacteria compared to solid media (e.g., Middlebrook 7H10 and 7H11).
2. Enriched with Inorganic Compounds
   • Supplies potassium, sulfur, magnesium, and phosphorus, which are essential for Mycobacterial metabolism.
3. Middlebrook ADC Supplement Compatibility
   • Addition of ADC Growth Supplement (bovine albumin, dextrose, catalase) enhances growth by:
     • Providing energy through dextrose.
     • Protecting Mycobacteria from toxic agents using albumin.
     • Neutralizing toxic peroxides with catalase.
4. Adaptable to Specific Growth Requirements
   • Glycerol or TWEEN® 80 can be added to optimize growth and energy supply.
5. Ideal for Drug Susceptibility Testing
   • Widely used for sensitivity testing of Mycobacterium tuberculosis.
6. Wide Range of Applications
   • Suitable for use in clinical diagnostics, research, food testing, and veterinary microbiology.

Specifications

Composition (per liter)

Preparation Instructions

1. Suspend 2.35 g of Middlebrook 7H9 Broth Base in 450 mL of distilled water.
2. Add either:
   • 2 mL of glycerol (Cat. No. 49767)
   • OR 0.5 g of TWEEN® 80 (Cat. No. 93780).
3. Heat gently to dissolve the medium completely.
4. Sterilize by autoclaving at 121°C for 10 minutes under 15 lbs. pressure.
5. Cool to 45°C or below.
6. Aseptically add 1 vial of Middlebrook ADC Growth Supplement (Cat. No. M0553).
7. Mix thoroughly before dispensing into sterile tubes or bottles.

Applications

1. Primary Isolation and Cultivation
   • Supports the isolation and cultivation of slow-growing and fastidious Mycobacterium tuberculosis and other Mycobacteria species.
2. Drug Susceptibility Testing
   • Ideal medium for antibiotic sensitivity testing of Mycobacterium tuberculosis to evaluate resistance to antimicrobials.
3. Large-Scale Propagation
   • Used for bulk cultivation of Mycobacterium bovis, M. avium, and M. smegmatis for research and vaccine production.
4. Clinical Diagnostics
   • Commonly applied in laboratories for liquid culture-based diagnosis of tuberculosis (TB).
5. Research Studies
   • Suitable for Mycobacterial metabolic studies, vaccine trials, and bacterial count assays in animal models.

Advantages

1. Rapid Growth of Mycobacteria
   • Mycobacteria grow more quickly in liquid media compared to solid media like Middlebrook 7H10 or 7H11.
2. Supports Sensitivity Testing
   • Allows evaluation of Mycobacterial resistance to antimicrobials in a liquid medium.
3. Nutrient-Rich and Versatile
   • Compatible with glycerol or TWEEN® 80 for enhanced energy supply.
4. High-Quality Growth Support
   • Addition of ADC Supplement ensures optimal nutrient availability and toxin protection.
5. Stable and Reliable
   • Consistent and reproducible performance for clinical and research applications.

Quality Control

Comparison to Other Middlebrook Media

The Middlebrook 7H9 Broth Base is a versatile, reliable, and nutrient-rich medium optimized for the rapid growth, cultivation, and sensitivity testing of Mycobacterium species. It is an essential tool for clinical diagnostics, research, and large-scale propagation.