Liofilchem™ MTS™ Imipenem IMI 0.002-32, MIC Test strips
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Specifications:
Application | Clinical microbiology |
Storage Temperature | -20°C |
Product Type | MIC Strips |
Product Brand | Liofilchem |
Product Grade | Microbiology grade |
The Liofilchem™ MTS™ Imipenem IMI 0.002-32 MIC Test Strips are designed for precise Minimum Inhibitory Concentration (MIC) determination of imipenem against a broad range of microorganisms. These strips are ideal for detecting carbapenem resistance mechanisms in clinical and research laboratories, especially in Gram-negative bacteria.
Key Features
- Broad MIC Range:
- Covers concentrations from 0.002 μg/mL to 32 μg/mL, suitable for highly resistant strains.
- Ease of Use:
- Simple application on Mueller-Hinton Agar.
- Accurate Results:
- Provides clear MIC endpoints within 16–20 hours of incubation.
- Global Standards:
- Compliant with CLSI, EUCAST, and other international guidelines.
- Resistance Detection:
- Effectively identifies carbapenemase-producing Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter spp.
Applications
Application | Details |
---|---|
Clinical Diagnostics | Identification of imipenem-resistant pathogens. |
Resistance Mechanism Detection | Screening for carbapenemase production in Gram-negative bacteria. |
Epidemiological Studies | Surveillance of antimicrobial resistance trends. |
Pharmaceutical Research | Assessment of new carbapenem antibiotics. |
Testing Procedure
- Preparation:
- Prepare a 0.5 McFarland standard suspension of the test organism.
- Inoculate the suspension evenly on a Mueller-Hinton Agar plate.
- Application:
- Place the Liofilchem Imipenem MIC Test Strip on the agar surface using sterile forceps.
- Incubation:
- Incubate the plate aerobically at 35–37°C for 16–20 hours.
- Interpretation:
- Determine the MIC by observing the point where the elliptical zone of inhibition intersects the strip.
Technical Specifications
Feature | Details |
---|---|
Active Compound | Imipenem |
Concentration Range | 0.002–32 μg/mL |
Compatible Media | Mueller-Hinton Agar |
Incubation Conditions | 35–37°C for 16–20 hours |
Storage | -20°C (long-term); 2–8°C (short-term) |
Shelf Life | Until expiration date on packaging |
Pack Sizes | 10, 30, 50, or 100 strips per pack |
Quality Control Data
Control Organism | Expected MIC Range (μg/mL) |
---|---|
Escherichia coli ATCC® 25922 | 0.03–0.12 |
Pseudomonas aeruginosa ATCC® 27853 | 1–4 |
Klebsiella pneumoniae ATCC® BAA-1705 (KPC-positive) | ≥16 |
Acinetobacter baumannii ATCC® 19606 | ≥8 |
Interpretation of Results
MIC Value | Interpretation |
---|---|
≤1 μg/mL | Susceptible |
2–4 μg/mL | Intermediate |
≥8 μg/mL | Resistant |
Refer to CLSI/EUCAST guidelines for specific interpretation criteria.
Advantages
Feature | Benefit |
---|---|
Wide Concentration Range | Detects resistance across a broad spectrum. |
High Sensitivity | Accurate identification of resistant isolates. |
Ease of Use | Simplifies MIC determination with clear visual results. |
Standards Compliance | Adheres to CLSI and EUCAST testing protocols. |
Comparison with Alternative MIC Testing Methods
Method | Liofilchem™ MTS™ Imipenem | Broth Microdilution | Disk Diffusion |
---|---|---|---|
Ease of Use | Easy | Labor-intensive | Easy but less precise |
Time to Results | 16–20 hours | 16–20 hours | 16–20 hours |
Quantitative MIC Value | Yes | Yes | No |
Resistance Detection | High sensitivity | High sensitivity | Moderate sensitivity |
Conclusion
The Liofilchem™ MTS™ Imipenem IMI 0.002-32 MIC Test Strips are a reliable and efficient solution for detecting imipenem resistance. Their precision, ease of use, and adherence to global standards make them indispensable for clinical laboratories, research institutions, and pharmaceutical companies monitoring antimicrobial resistance.
- Pack Size: 100/pk 10/pk 30/pk