Liofilchem™ MTS™ Azithromycin AZM 0.016-256, MIC Test strips

The Liofilchem™ MTS™ Azithromycin MIC Test Strips are an advanced diagnostic tool designed to determine the minimum inhibitory concentration (MIC) of azithromycin against bacterial pathogens. These gradient-based test strips provide precise MIC values, helping clinicians and microbiologists evaluate the susceptibility of microorganisms to azithromycin for effective antimicrobial therapy.

Key Features:

1. Wide MIC Range:
   • Detects azithromycin MIC values from 0.016 to 256 µg/mL, covering a broad range of bacterial susceptibility.
2. Gradient Technology:
   • Creates a continuous azithromycin concentration gradient along the strip, enabling precise MIC determination.
3. Ease of Use:
   • Pre-calibrated and ready-to-use strips eliminate preparation time, ensuring accurate and reproducible results.
4. High Quality:
   • Manufactured under strict quality control standards, ensuring compliance with CLSI and EUCAST guidelines for susceptibility testing.
5. Versatile Application:
   • Suitable for gram-positive and gram-negative bacterial pathogens, including those causing respiratory, skin, and sexually transmitted infections.

Applications:

1. Clinical Diagnostics:
   • Determines the MIC of azithromycin for guiding appropriate antibiotic therapy.
2. Antimicrobial Resistance Surveillance:
   • Monitors resistance patterns in pathogens to support public health efforts.
3. Pharmaceutical Testing:
   • Evaluates the efficacy of azithromycin formulations during drug development and quality control.
4. Research Microbiology:
   • Studies resistance mechanisms and evaluates azithromycin efficacy in experimental models.

Use Cases:

1. Respiratory Infections:
   • Tests MIC for pathogens like Streptococcus pneumoniae and Haemophilus influenzae.
2. Skin and Soft Tissue Infections:
   • Evaluates susceptibility of Staphylococcus aureus (including MRSA) to azithromycin.
3. Sexually Transmitted Infections (STIs):
   • Determines MIC for Neisseria gonorrhoeae and Chlamydia trachomatis.
4. Enteric Pathogens:
   • Assesses azithromycin activity against Salmonella spp. and Shigella spp..

Specifications:

Testing Procedure:

1. Preparation:
   • Prepare a Mueller-Hinton agar plate and inoculate with a standardized bacterial suspension (e.g., 0.5 McFarland standard).
2. Strip Application:
   • Place the MTS™ Azithromycin strip onto the agar surface using sterile forceps or an applicator.
3. Incubation:
   • Incubate the plate at 35°C for 16–20 hours in an aerobic atmosphere.
4. MIC Reading:
   • Observe the ellipse of growth inhibition intersecting the strip.
   • Read the MIC value at the point where the inhibition ellipse touches the strip.

Advantages:

1. Precise MIC Values:
   • Gradient technology ensures accurate determination of azithromycin susceptibility.
2. User-Friendly:
   • Easy to apply and interpret with minimal training.
3. Broad Applicability:
   • Suitable for a wide range of bacterial species and clinical scenarios.
4. Reproducible Results:
   • High-quality manufacturing ensures consistent and reliable results across batches.

Storage and Handling:

• Store at 2°C to 8°C in a dry environment, away from light and moisture.
• Avoid prolonged exposure to room temperature to maintain strip potency.
• Use strips before the labeled expiration date.

Product Details:

Pathogens Typically Tested:

• Streptococcus pneumoniae
• Haemophilus influenzae
• Staphylococcus aureus (including MRSA)
• Neisseria gonorrhoeae
• Salmonella spp.
• Shigella spp.

Quality Control:

• Control Strains:
  • Escherichia coli ATCC® 25922 (susceptible).
  • Staphylococcus aureus ATCC® 29213 (susceptible).
  • Haemophilus influenzae ATCC® 49247 (susceptible).
• Routine quality control ensures compliance with CLSI and EUCAST standards.

The Liofilchem™ MTS™ Azithromycin (AZM) MIC Test Strips, 0.016–256 µg/mL offer a precise and reliable method for determining azithromycin susceptibility in clinical, research, and industrial microbiology applications. Their gradient technology and broad-spectrum utility make them indispensable for guiding antimicrobial therapy and monitoring resistance trends.