Liofilchem™ Blood AD Clindamycin 0.03-32, Agar Dilution AST

The Liofilchem™ Blood AD Clindamycin 0.03-32, Agar Dilution Antimicrobial Susceptibility Testing (AST) system is a high-precision method for determining the Minimum Inhibitory Concentration (MIC) of Clindamycin in bacterial isolates from blood cultures. This method utilizes agar dilution to evaluate bacterial susceptibility to Clindamycin, a lincosamide antibiotic used for treating infections caused by Gram-positive bacteria and anaerobes.

Key Features

1. Wide MIC Range

• Covers a range from 0.03 to 32 μg/mL, providing comprehensive detection of both low- and high-level resistance.

2. High Sensitivity

• Designed to accurately assess Clindamycin susceptibility in blood culture isolates, including:
  • Staphylococcus aureus (MRSA and MSSA).
  • Streptococcus spp.
  • Anaerobic bacteria like Clostridium spp.

3. Standardized Testing

• Fully compliant with international guidelines, including EUCAST and CLSI, ensuring reliable and reproducible results.

4. Precision and Reliability

• Agar dilution offers direct observation of bacterial growth, delivering consistent MIC values for clinical and research applications.

5. Application-Specific

• Specifically tailored for isolates from bloodstream infections, critical for guiding effective treatment.

6. Quality Manufacturing

• Produced under strict quality control standards to ensure reproducible results and consistent performance.

Applications

1. Clinical Diagnostics

• Determine the susceptibility of pathogens isolated from blood cultures to Clindamycin.

2. Inducible Resistance Detection

• Used alongside erythromycin in a D-test to detect inducible resistance to Clindamycin in Staphylococcus aureus and Streptococcus spp.

3. Resistance Surveillance

• Monitor Clindamycin resistance trends in healthcare settings, particularly for bloodstream infections.

4. Research and Development

• Evaluate antimicrobial efficacy and study resistance mechanisms in bacterial isolates from blood cultures.

Procedure

1. Preparation of Agar Plates

• Prepare Mueller-Hinton agar plates supplemented with serial dilutions of Clindamycin (0.03–32 μg/mL).

2. Inoculation

• Prepare bacterial suspensions standardized to 0.5 McFarland.
• Spot-inoculate 1–2 μL of suspension onto each concentration of Clindamycin in the agar.

3. Incubation

• Incubate plates at 35–37°C for 16–20 hours under aerobic or anaerobic conditions, depending on the organism.

4. Result Interpretation

• Determine the MIC as the lowest Clindamycin concentration that inhibits visible bacterial growth.

Specifications

Advantages

1. Precise and Reliable

• Direct MIC determination via visual assessment of bacterial growth.

2. Broad Applicability

• Effective for testing both aerobic and anaerobic bacteria isolated from blood cultures.

3. Resistance Detection

• Enables detection of both constitutive and inducible clindamycin resistance.

4. Tailored for Bloodstream Infections

• Specifically designed for critical diagnostic applications involving bloodstream isolates.

Limitations

• Labor-intensive compared to automated or gradient diffusion methods.
• Requires technical expertise for proper preparation, inoculation, and interpretation.

Why Choose Liofilchem™ Blood AD Clindamycin 0.03-32, Agar Dilution AST?

• Accuracy: Provides precise and reproducible MIC values for clinical decision-making.
• Versatility: Covers a broad range of pathogens and resistance levels.
• Reliability: Fully compliant with international standards, ensuring consistent results.

The Liofilchem™ Blood AD Clindamycin 0.03-32, Agar Dilution AST system is an indispensable tool for clinical laboratories and research institutions, delivering robust, accurate, and standardized testing for bloodstream infections.