Liofilchem® Tween 80 Supplement

The Liofilchem Tween 80 Supplement is a polysorbate-based additive designed for the enrichment of culture media. It is widely used to support rapid bacterial growth and is essential for preparing specific media such as MRS Agar, Rogosa Agar, and other microbiological growth mediums.

Key Features:

1. Promotes Bacterial Growth:
   • Enhances the growth of lactic acid bacteria and other microorganisms in enriched culture media.
2. Broad Compatibility:
   • Compatible with multiple culture media, including HHD Broth, MRS Agar, Letheen Agar, Rogosa Agar, and more.
3. Convenient and Ready-to-Use:
   • Supplied as a 50 mL concentrated solution for direct addition to culture media before sterilization.
4. Easy Sterilization:
   • Fully compatible with autoclave sterilization protocols.
5. Reliable and Consistent:
   • Manufactured under strict quality controls to ensure consistency and efficacy.

Composition:

Applications:

1. Microbial Growth Media:
   • Enhances bacterial growth in media such as MRS Agar, Rogosa Agar, and Letheen Broth.
2. Food and Dairy Testing:
   • Commonly used in microbiological analysis of food and dairy products.
3. Research and Quality Control:
   • Essential in laboratory research and industrial testing involving lactic acid bacteria.

Protocol:

1. Preparation:
   • Refer to the specific culture medium instructions for the recommended amount of Tween 80 Supplement to be added.
   • Mix the supplement thoroughly with the medium.
2. Sterilization:
   • Distribute the prepared medium into containers and sterilize in an autoclave as per the medium’s instructions.
3. Application:
   • Use the sterilized medium for bacterial cultivation and enumeration.

Quality Control:

Specifications:

Product Variants:

Advantages:

1. Enhanced Growth:
   • Promotes robust bacterial growth, ensuring reliable results in microbiological analyses.
2. Versatile Usage:
   • Applicable in various media formulations, making it a flexible solution for microbiologists.
3. Simple Storage:
   • Stable at refrigerated temperatures, maintaining performance until the stated expiration date.
4. High-Quality Standards:
   • Consistently manufactured to meet stringent laboratory requirements.

Storage and Handling:

• Store at 2–8°C in its original packaging.
• Do not use beyond the labeled expiry date.
• Discard if signs of deterioration or contamination are evident.

Safety and Precautions:

• For professional use only.
• Operators must be trained in laboratory methods.
• Refer to the Safety Data Sheet (SDS) for information on hazards and safe handling.

References:

1. De Man, J.C., Rogosa, M., and Sharpe, M.E. (1960). J. Appl. Bact. 23:130-135.
2. ISO 15214 (1998). Microbiology of food and animal feeding stuffs – Enumeration of mesophilic lactic acid bacteria.
3. Briggs, M. (1953). J. Dairy Res. 20: 36.

The Liofilchem® Tween 80 Supplement is a reliable and essential additive for preparing enriched culture media, ensuring the robust growth of microorganisms in various microbiological applications. Its compatibility, ease of use, and quality make it indispensable for laboratories and quality control settings.