Liofilchem® HEMO-AEROBIC Blood Culture Bottles

Liofilchem® HEMO-AEROBIC CULTURING is a diagnostic device designed for the isolation and cultivation of fastidious, aerobic, and facultative anaerobic microorganisms from venous blood. The bottles contain Brain Heart Infusion Broth (BHI) enriched with p-Aminobenzoic Acid (PABA) and Sodium-polyanethol-sulphonate (SPS), enhancing the growth of microorganisms while neutralizing inhibitors and preventing blood coagulation.

Product Variants

1. HEMO-AEROBIC CULTURING (For Adults)
   • Volume: 80 mL per bottle.
2. HEMO-AEROBIC CULTURING Pediatric (For Children)
   • Volume: 40 mL per bottle.
3. HEMO-AEROBIC CULTURING Neonatal (For Newborns and children under 2 years)
   • Volume: 9 mL per bottle.

Each variant is tailored to meet specific clinical needs based on patient age and sample volume requirements.

Key Features

• Nutrient-rich Medium: Brain Heart Infusion Broth supplemented with PABA and SPS supports optimal growth of bloodborne microorganisms.
• Anticoagulant Properties: SPS prevents blood clotting and neutralizes bactericidal effects of human serum.
• Wide Microbial Support: Suitable for isolating a wide variety of bacteria, including Gram-positive, Gram-negative, and fungi.
• Convenient Packaging: Includes bottles with protective caps to ensure sterility and ease of handling.

Medium Composition (g/L)

• Brain Heart Infusion: 17.0
• Peptone: 10.0
• Dextrose: 2.0
• Sodium Chloride: 5.0
• Sodium Phosphate Bibasic: 2.5
• p-Aminobenzoic Acid (PABA): 0.05
• Sodium-polyanethol-sulphonate (SPS): 0.3
• Final pH: 7.4 ± 0.2

Clinical Applications

• Isolation and identification of pathogens in bloodstream infections (BSI).
• Detection of aerobic and facultative anaerobic microorganisms in cases of septicemia and other infections.
• Monitoring of bloodstream infections in pediatric and neonatal patients.

How It Works

1. Sample Collection:
   • Venous blood is drawn aseptically and inoculated into the bottle.
   • Adult samples: 8-10 mL, pediatric samples: 4-5 mL, neonatal samples: 0.5-1 mL.
2. Inoculation and Incubation:
   • Blood is mixed with the medium to ensure even distribution.
   • Incubate the bottles at 36 ± 1°C for up to 7 days.
3. Subculturing:
   • Periodically check for microbial growth by subculturing on selective agar plates.
   • Perform Gram staining and microscopic examination to identify microbial morphology.

Advantages

• Broad Spectrum: Supports the growth of fastidious organisms, including Streptococcus pneumoniae, which may undergo autolysis without appropriate subculturing.
• Inhibitor Neutralization: PABA neutralizes the effects of sulfonamides, ensuring accurate microbial recovery.
• Enhanced Anticoagulant Properties: SPS prevents coagulation, minimizes serum bactericidal effects, and partially inactivates certain antibiotics like streptomycin, kanamycin, and gentamicin.

Quality Control

Each batch undergoes quality control using the following reference strains:

• Escherichia coli ATCC® 25922
• Staphylococcus aureus ATCC® 25923
• Klebsiella pneumoniae ATCC® 13883
• Haemophilus parainfluenzae ATCC® 7901
• Pseudomonas aeruginosa ATCC® 27853
• Candida albicans ATCC® 10231
• And other standard strains.

Limitations

• Fastidious Microorganisms: Some strains may not grow in the specified incubation period.
• Inhibitors: Blood samples containing antibiotics may reduce microbial growth.
• False Negatives: Early disposal of bottles may lead to missed detections.

Safety and Storage

• Storage Conditions: Store at 10-25°C in original packaging.
• Shelf Life: Valid until the expiry date on the label.
• Disposal: Follow laboratory protocols for the decontamination and disposal of biohazardous material.

Ordering Information

References

1. Murray P.R., Manual of Clinical Microbiology, 7th Ed. (2005). ASM Press.
2. Finegold S.M., Diagnostic Microbiology, 7th Ed. (1986). C.V. Mosby Co.

Liofilchem® products are manufactured with the highest standards of quality to ensure reliable diagnostic outcomes.