Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X), 20 mL

Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X) is a ready-to-use loading buffer designed for preparing protein samples for denaturing SDS-PAGE using Tris-Glycine gels. With a pH of 6.8, this buffer includes sodium dodecyl sulfate (SDS) for protein denaturation and bromophenol blue as a visual tracking dye during electrophoresis.

It is optimized to ensure consistent protein migration and compatibility with common detection methods such as Coomassie staining, silver staining, and Western blotting. Its dual-use design allows for reduced or non-reduced sample preparation, depending on your workflow needs.

Key Features and Benefits

• 2X concentration – simple dilution for immediate use
• Includes SDS and bromophenol blue – for effective protein denaturation and run monitoring
• Optimized pH 6.8 – matches Tris-Glycine gel systems
• Heat-stable – best performance when samples are heated at 85°C for 2–5 minutes
• Versatile – use with reduced or non-reduced samples
• Avoids proteolysis – lower heating temperatures reduce protein degradation

Usage Guidelines

• Dilute 1:1 with your protein sample to achieve a 1X working concentration
• Heat the sample at 85°C for 2–5 minutes before loading
• Avoid heating above 90°C to minimize protein degradation in SDS-containing environments
• Compatible with Tris-Glycine gels for SDS-PAGE

Applications

• SDS-PAGE protein separation using Tris-Glycine gel systems
• Western blotting sample preparation
• Coomassie and silver-stain protein visualization
• General protein electrophoresis workflows

Novex™ Tris-Glycine SDS Sample Buffer (2X) is an essential reagent for any SDS-PAGE workflow using Tris-Glycine gel systems. With its easy-to-use formulation, optimized pH, and compatibility with denaturing conditions, it supports accurate and reproducible protein separation in research and routine lab settings.