Invitrogen™ Streptavidin, lyophilized powder

Invitrogen™ Streptavidin is a high-affinity biotin-binding protein purified from Streptomyces avidinii, designed for use in biotin-based detection, capture, and purification applications. Streptavidin shares strong biotin-binding properties with avidin, but exhibits significantly lower nonspecific binding, making it especially suited for applications requiring enhanced specificity and minimal background.

Each milligram of streptavidin delivers binding activity >11 units, where one unit binds 1 µg of biotin, as originally defined by Green (Biochem. J., 89:599, 1963). The protein is lyophilized from 10 mM PBS (pH 7.4) and can be reconstituted in distilled or deionized water for immediate use.

Key Features and Benefits

• High Biotin Affinity: Strong, non-covalent interaction (Kd ≈ 10⁻¹⁵ M) ideal for sensitive detection assays
• Low Nonspecific Binding: Reduced background interference compared to avidin
• Lyophilized Form: Long shelf-life, easy reconstitution with 1 mL water
• Unconjugated: Ready for use in direct binding, coating, conjugation, or amplification systems
• Reliable Activity: ≥11 units/mg biotin-binding capacity

Applications

• Biotin-Streptavidin Capture Systems
  Use in ELISA, western blotting, or immunoprecipitation for capturing biotinylated molecules
• Fluorescence & Enzyme Labeling
  Base protein for conjugation to fluorophores (e.g., FITC), enzymes (e.g., HRP, AP), or nanoparticles
• Surface Coating in Assay Platforms
  Coating for biosensors, bead-based assays, and microplate surfaces
• Affinity Purification
  Capture of biotinylated proteins, DNA, or ligands from complex mixtures
• Molecular Imaging and Detection
  Integration into streptavidin-based labeling and targeting strategies

Technical Specifications

Invitrogen™ Streptavidin, Lyophilized Powder provides a highly pure, biotin-binding reagent that offers both strong affinity and low background for biotin-streptavidin applications. Whether you're performing biomolecule detection, conjugation, or surface capture, this formulation ensures reliable and reproducible performance in molecular biology and biochemical research workflows.