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Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwich, 8.3 x 7.3 cm
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Specifications:
| Application | Western Blot |
| Storage Temperature | Room Temperature |
| Product Type | Filter Papers |
| Product Brand | Thermo Fisher Scientific™, Invitrogen |
| Product Grade | Molecular Biology |
Invitrogen™ PVDF/Filter Paper Sandwiches provide an all-in-one solution for efficient protein and nucleic acid transfer in Western blotting, dot blotting, and sequencing applications. Each unit includes a high-performance polyvinylidene difluoride (PVDF) membrane layered with absorbent filter paper, pre-cut and pre-assembled for mini gel systems.
- LC2002 (0.2 μm pore size) is optimized for transferring low molecular weight proteins and peptides, providing high binding efficiency even with minimal sample.
- LC2005 (0.45 μm Invitrolon™ version) offers broad protein compatibility and mechanical strength, making it suitable for repeated stripping and probing.
Both formats ensure consistent, high-sensitivity results across a wide range of blotting techniques.
Key Features and Benefits
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Two precision formats:
- 0.2 μm: Optimal for low MW proteins, peptides, and amino acid sequencing
- 0.45 μm: Ideal for routine protein transfer and western blotting workflows
- Pre-assembled convenience: Each sandwich includes PVDF membrane + filter paper, saving prep time
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High binding capacity:
- 0.2 μm: ~50–150 µg/cm² (higher for small peptides)
- 0.45 μm: ~294 µg/cm² for large proteins
- Robust and reusable: High mechanical strength allows for multiple stripping and reprobing cycles
- Chemical compatibility: Resistant to organic solvents, acids, and mild bases
- Universal detection: Compatible with chemiluminescence, fluorescence, radiolabeling, and colorimetric detection methods
- Required pre-activation: Hydrate with 100% methanol, ethanol, or isopropanol before use
Specifications
| Attribute | LC2002 (0.2 μm) | LC2005 (0.45 μm) |
|---|---|---|
| Membrane Type | PVDF | Invitrolon™ PVDF |
| Pore Size | 0.2 μm | 0.45 μm |
| Dimensions (W × L) | 8.3 × 7.3 cm | 8.3 × 7.3 cm |
| Format | Pre-cut membrane/filter paper sandwich | Pre-cut membrane/filter paper sandwich |
| Quantity per Pack | 20 sandwiches | 20 sandwiches |
| Binding Capacity | 50–150 μg/cm² (proteins & peptides) | ~294 μg/cm² (IgG) |
| Reprobing Capability | Yes | Yes |
| Filter Paper Thickness | 0.8 mm | 0.8 mm |
| Recommended Applications | Peptide transfer, protein sequencing | Routine Western blotting |
| Pre-activation Required | Yes | Yes |
| Storage Conditions | Room temperature | Room temperature |
Applications
- Western blotting for proteins of various molecular weights
- Dot and slot blot assays
- Amino acid sequencing and MS prep (LC2002)
- Protein transfer from SDS-PAGE gels
- Multiple probe studies with stripping/reprobing
Invitrogen™ PVDF/Filter Paper Sandwiches in mini format offer fast, consistent, and high-sensitivity performance for protein and nucleic acid transfer. Choose LC2002 for low MW and peptide detection, or LC2005 for general-purpose Western blotting using Invitrolon™ PVDF. Pre-cut and pre-assembled, they ensure lab-ready convenience without sacrificing membrane performance.
- Pore Size: 0.45 µm 0.2 µm