Invitrogen™ eBioscience™ Cell Stimulation Cocktail (500X)

The Invitrogen™ eBioscience™ Cell Stimulation Cocktail (500X) is a potent reagent containing a combination of phorbol 12-myristate 13-acetate (PMA) and ionomycin. This cocktail is designed to activate various cell types, inducing cytokine production for downstream detection in immunological assays such as flow cytometry, ELISA, and functional assays. It can also be paired with the Protein Transport Inhibitor Cocktail (500X) (Cat. 00-4980) to facilitate intracellular retention of cytokine expression for advanced intracellular staining.

Key Features:

1. Effective Cell Activation:
   • PMA activates protein kinase C (PKC) signaling, and ionomycin increases intracellular calcium levels, collectively driving robust activation of immune cells.
2. Induces Cytokine Production:
   • Stimulates various cell types, including T cells, to produce cytokines for subsequent analysis.
3. Flexible Applications:
   • Compatible with multiple assay formats, including:
     • Flow Cytometry
     • ELISA
     • Functional Assays
     • In Vitro Cell Culture
4. High Concentration:
   • Supplied as a 500X concentrated solution, ensuring ease of use and cost efficiency.
5. Optimized for Research:
   • Designed for reproducible results across a range of immunological research applications.

Specifications:

Applications:

1. Cytokine Induction:
   • Activates immune cells, such as T cells, for the production of cytokines like IL-2, IFN-γ, and others.
2. Flow Cytometry:
   • Stimulates cells for intracellular cytokine staining and multicolor flow cytometry panels.
3. ELISA and Immunoassays:
   • Induces cytokine production for detection and quantification in enzyme-linked immunosorbent assays (ELISA).
4. In Vitro Functional Assays:
   • Used in functional studies to analyze cell activation and response to stimuli.
5. Intracellular Retention:
   • Can be paired with Protein Transport Inhibitor Cocktail to retain cytokines intracellularly during flow cytometric analysis.

Key Benefits:

1. High Efficacy:
   • PMA and ionomycin work synergistically to ensure robust activation of cells.
2. Simplified Workflow:
   • Highly concentrated formulation (500X) reduces reagent use and simplifies preparation.
3. Versatility:
   • Compatible with a wide range of cell types and assays, making it suitable for various immunological studies.
4. Reproducible Results:
   • Provides consistent performance for reliable and reproducible data.

Usage Instructions:

1. Dilute the 500X solution to the desired working concentration in cell culture medium.
2. Add to cells in vitro to stimulate cytokine production.
3. Incubate for the recommended time, depending on the assay type.
4. For intracellular retention of cytokines, pair with the Protein Transport Inhibitor Cocktail.

Storage and Handling:

• Storage Conditions: Store at 2–8°C for stability.
• Handling: Protect from light and avoid repeated freeze-thaw cycles to maintain reagent integrity.

For Research Use Only:

This product is intended for research purposes only and is not suitable for diagnostic or therapeutic applications.