Invitrogen™ Dynabeads™ Untouched™ Human CD4 T Cells Kit

The Invitrogen™ Dynabeads™ Untouched™ Human CD4 T Cells Kit enables the isolation of pure and viable CD4+ T cells from peripheral blood mononuclear cells (PBMCs) using a negative isolation approach. By depleting non-CD4 cells, the kit leaves untouched CD4+ T cells free of bead and antibody contact, ensuring their functionality and compatibility with downstream applications such as flow cytometry and functional assays.

Key Features:

1. Untouched CD4+ T Cells:
   • Isolated cells remain free of beads and antibodies, preserving their natural state and functionality.
2. High Purity and Viability:
   • Achieves highly pure, viable CD4+ T cells, suitable for sensitive downstream applications.
3. Negative Isolation Technology:
   • Depletes non-CD4 cells, including CD8+ T cells, B cells, NK cells, monocytes, platelets, granulocytes, dendritic cells, and erythrocytes.
4. Easy-to-Use and Scalable:
   • No columns required; scalable for different input volumes and sample sizes.
5. Fast and Efficient Protocol:
   • Short incubation times for both antibody binding and bead depletion streamline the workflow.

Specifications:

Kit Components:

Antibody Mix includes monoclonal antibodies targeting CD8, CD14, CD16 (a and b), CD19, CD36, CD56, CD123, and CD235a (Glycophorin A).

Applications:

1. Flow Cytometry:
   • Directly analyze untouched CD4+ T cells for surface markers, activation, and differentiation.
2. Functional Assays:
   • Use CD4+ T cells for proliferation, cytokine secretion, and immune response studies.
3. Immunological Research:
   • Investigate CD4+ T cell subsets, including helper and regulatory T cells.
4. Translational Studies:
   • Evaluate CD4+ T cell roles in autoimmune diseases, infectious diseases, and cancer.

Isolation Procedure:

1. Labeling Non-CD4 Cells:
   • Add the antibody mix to PBMCs and incubate for a short time to label non-CD4 cells.
2. Binding Dynabeads™:
   • Add Dynabeads™ to bind antibody-labeled non-CD4 cells.
3. Magnetic Separation:
   • Place the sample in a magnetic separator to retain bead-bound cells. The untouched CD4+ T cells remain in the supernatant.
4. Collect CD4+ T Cells:
   • Transfer the CD4+ T cell-containing supernatant for immediate use or downstream applications.

Advantages:

1. Preserved Cell Functionality:
   • Negative isolation ensures no direct contact with beads or antibodies, maintaining natural cell characteristics.
2. Scalable and Flexible:
   • Adapts to various sample sizes and input volumes, accommodating small or large-scale experiments.
3. High Recovery Rates:
   • Efficient depletion of non-CD4 cells results in high CD4+ T cell purity and yield.
4. Simple and Fast Protocol:
   • Eliminates the need for columns, reducing complexity and processing time.

Storage and Handling:

• Storage Conditions: Store kit components at 2–8°C. Avoid freezing.
• Shelf Life: Refer to product labeling for expiration date.

Ordering Information:

The Invitrogen™ Dynabeads™ Untouched™ Human CD4 T Cells Kit provides a robust, efficient, and reliable solution for isolating untouched CD4+ T cells. Its ability to deliver high-purity, bead-free cells makes it ideal for functional assays, immunological research, and translational studies.