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Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit
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Specifications:
| Application | PCR | ||
| Storage Temperature | Room Temperature | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit offers a fast, ethanol-free, and highly efficient solution for purifying DNA fragments from PCR reactions. Unlike conventional silica-based purification methods, the ChargeSwitch™-Pro kit uses an innovative ion-exchange mechanism that eliminates the need for ethanol, chaotropic salts, or organic solvents—substances known to interfere with downstream enzymatic reactions.
This unique chemistry allows DNA to bind at low pH and elute at pH 8.5, producing inhibitor-free DNA with superior quality for downstream applications. The kit provides a 20% reduction in handling steps, simplifying workflows and reducing variability while ensuring high yields of pure DNA.
Key Features
- Ethanol-Free, Chaotrope-Free – Avoids common inhibitors of enzymatic reactions.
- Improved Downstream Performance – Enhances sequencing, cloning, and digestion efficiency.
- Fast and Simple Protocol – 20% fewer handling steps than standard methods.
- High Recovery – Purifies up to 500 μL of PCR product per column.
- Complete Kit – Includes columns, collection tubes, recovery tubes, and all necessary buffers.
- Sustainable Workflow – Reduced hazardous reagents and waste generation.
How It Works
The ChargeSwitch™ ion-exchange chemistry binds DNA to a positively charged surface ligand at low pH and elutes it when the pH is raised to 8.5. This avoids harsh reagents used in traditional PCR clean-up and produces DNA free of enzymatic inhibitors, improving the reliability of downstream workflows such as:
- PCR amplification
- Restriction enzyme digestion
- Sequencing (including array CGH labeling)
- Cloning
Applications
- PCR product purification for cloning and ligation workflows.
- Removal of primers, dNTPs, polymerases, and salts.
- DNA preparation for next-generation sequencing (NGS).
- Downstream enzymatic assays including restriction digestion.
- Research workflows requiring inhibitor-free DNA.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | PCR Clean-Up Kit |
| Catalog Numbers | CS32050 (50 preps), CS32250 (250 preps) |
| Sample Size | Up to 500 μL PCR product |
| Format | Spin columns |
| Run Time | Fast protocol with 20% fewer steps |
| Technology | ChargeSwitch™ ion-exchange chemistry |
| Applications | PCR, NGS, cloning, sequencing |
| Target | DNA from enzymatic reactions |
| High-Throughput | Manual use only (not automation compatible) |
| Green Features | Reduced hazardous chemicals, fewer resources used |
| Quantity | 50 or 250 preps |
| Shipping Condition | Room temperature |
| Storage Condition | Room temperature, stable for 6 months |
Kit Contents
- ChargeSwitch™-Pro Columns
- Collection tubes
- Recovery tubes
- Binding buffer
- Wash buffer
- Elution buffer
Unique Advantages
- Provides clean, inhibitor-free DNA for maximum reliability.
- Outperforms traditional silica column kits in sequencing and cloning.
- Safe and environmentally responsible with fewer hazardous reagents.
- Optimized for manual workflows, balancing speed and efficiency.
The Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit sets a new standard for DNA purification by combining simplicity, speed, and improved downstream performance. With its ethanol-free, chaotrope-free chemistry, it delivers pure DNA that enhances cloning, sequencing, and enzymatic reactions. Designed for researchers who need reliable, high-quality DNA purification, it provides an effective and eco-friendly alternative to conventional PCR clean-up kits.
- Pack Size: For 50 preps For 50 Preps