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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ Anza™ 14 SalI is a high-efficiency restriction enzyme that recognizes the sequence 5′ G^TCGAC 3′ / 3′ CAGCT^G 5′, generating 5′ overhangs. It provides complete digestion in 15 minutes at 37 °C, while maintaining flexibility for extended incubations of up to 16 hours without star activity.
As part of the Anza™ Restriction Enzyme System, it operates in a universal single-buffer format (Anza Buffer), allowing seamless combination of multiple enzymes in a single reaction without sequential digestion or DNA clean-up steps. For added convenience, Anza Red Buffer is also supplied, incorporating tracking dyes and density reagent for direct loading onto gels.
Key Features
- Universal single buffer – all Anza enzymes function in the same buffer for simplified workflows.
- Fast digestion – complete DNA digestion in as little as 15 minutes.
- Flexible performance – overnight digestions possible without star activity.
- Streamlined protocol – one protocol suitable for all DNA types.
- Cloning-ready system – compatible with Anza DNA Modifying Enzymes for complete cloning workflows.
- Convenient Red Buffer option – direct gel loading with built-in tracking dyes.
Applications
- Molecular cloning workflows.
- Restriction site mapping.
- Genotyping and SNP analysis.
- Southern blotting.
- RFLP (restriction fragment length polymorphism) analysis.
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | SalI |
| Recognition Sequence | 5′ G^TCGAC 3′ / 3′ CAGCT^G 5′ |
| Cleavage Product | 5′ protruding ends |
| Optimal Reaction Temperature | 37 °C |
| Complete Digestion Time | 15 minutes |
| Long Incubation Stability | Up to 16 hours without star activity |
| Compatible Buffers | Anza™ 10X Buffer, Anza™ 10X Red Buffer |
| Methylation Sensitivity | Sensitive to CpG methylation; not Dam/Dcm methylation-sensitive |
| Heat Inactivation | Yes |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Formats
| Catalog Number | Quantity | Unit Size | Format |
|---|---|---|---|
| IVGN0146 | 1,500 units | 10 U/µL | Enzyme + Buffers |
| IVGN0148 | 8,000 units | 10 U/µL | Enzyme + Buffers |
Contents include:
- Anza 14 SalI enzyme (400 µL)
- 1 mL Anza Buffer (10X)
- 1 mL Anza Red Buffer (10X)
Invitrogen™ Anza™ 14 SalI combines speed, convenience, and flexibility in DNA digestion, making it a reliable choice for molecular cloning and genotyping applications. With its universal buffer system, fast digestion times, and extended stability without star activity, it simplifies restriction workflows while supporting compatibility with downstream cloning enzymes. This ensures accuracy, reproducibility, and efficiency in modern molecular biology laboratories.
- Pack Size: 1,500 Units 8,000 Units