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Thermo Scientific™ Oxoid™ Biochemical Identification System (O.B.I.S.) PYR, 60 Tests
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Specifications:
| Application | Microbial Identification/AST | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Microbial ID System | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
Rapid, Colorimetric Detection of PYRase Activity in Streptococci and Citrobacter spp.
The Thermo Scientific™ O.B.I.S. PYR is a rapid colorimetric test designed for the detection of PYRase (pyrrolidonyl peptidase) activity in streptococci, enterococci, and Citrobacter spp. This test serves as a rapid diagnostic alternative to traditional culture-based identification methods, delivering clear and reliable results in just 6 minutes.
Utilizing non-carcinogenic substrates, O.B.I.S. PYR is an advanced and safer option for microbial enzymatic testing. It is particularly useful for differentiating Salmonella spp. from Citrobacter spp. and other Enterobacteriaceae in both clinical and food microbiology applications.
Key Features & Benefits
- ✅ Rapid Results – Visual color change within 6 minutes
- ✅ Safe & Non-Carcinogenic – Uses 7-amino-4-methylcoumarin (7AMC) instead of β-naphthylamide
- ✅ Simple Protocol – Easy-to-use test cards with minimal training required
- ✅ Distinct Color Change – PYR-positive organisms produce a purple color upon reaction
- ✅ Differentiation Utility – Helps distinguish Citrobacter spp. from Salmonella spp.
- ✅ Includes Reagents – Complete kit includes test cards, buffer, and developing solution
- ✅ Compact Format – Ideal for both high- and low-throughput laboratories
Product Specifications
| Attribute | Details |
|---|---|
| Product Name | O.B.I.S. PYR |
| Catalog Number | ID0580M |
| Detectable Analyte | PYRase (Pyrrolidonyl peptidase) activity |
| Target Organisms | Streptococci, Enterococci, Citrobacter spp. |
| Formulation | Test cards impregnated with L-pyroglutamic acid 7AMC and dimethylaminocinnamaldehyde |
| Format | 60 Tests/Kit |
| Readout Time | ≤6 minutes |
| Detection Method | Colorimetric (purple indicates positive reaction) |
| Unit Size | Each |
| Kit Contents | Test cards, developing solution, buffer solution |
| Storage Conditions | 2°C to 8°C (Refrigerated) |
| Shipping Condition | Cold chain recommended |
| For Use With | Bacterial colonies on selective media (enteric or Gram-positive) |
| Intended Use | For research and microbiological identification purposes |
Applications
🧫 Clinical Microbiology – Rapid identification of Group A streptococci and enterococci
🔬 Food Microbiology – Differentiation of Salmonella spp. from Citrobacter spp. in food samples
🏥 Laboratory Diagnostics – Reliable, on-demand biochemical identification tool
🧪 Antimicrobial Stewardship Support – Enables targeted therapeutic decisions based on pathogen type
Ordering Information
| Catalog # | Description | Pack Size |
|---|---|---|
| ID0580M | O.B.I.S. PYR Rapid PYRase Test Kit | 60 Tests/Kit |
The Thermo Scientific™ O.B.I.S. PYR Test offers rapid, safe, and specific detection of PYRase activity in streptococci and Citrobacter spp., making it a valuable tool in clinical, food, and research microbiology. With its non-carcinogenic chemistry, fast results, and simple workflow, O.B.I.S. PYR ensures confidence in microbial identification while enhancing laboratory efficiency.