Human High Sensitivity C-Reactive Protein (hs-CRP) ELISA Kit

The Human hs-CRP ELISA Kit is a sensitive, sandwich-based enzyme-linked immunosorbent assay designed for the quantitative measurement of high sensitivity C-reactive protein (hs-CRP) in human serum, plasma, cell culture supernatants, tissue homogenates, and other biological fluids. hs-CRP is an acute-phase inflammatory biomarker commonly used in research related to cardiovascular disease, metabolic disorders, autoimmune conditions, and infection-associated inflammation.

This kit provides a robust platform for detecting minute changes in systemic CRP levels, with high sensitivity (0.1 ng/mL) and minimal sample volume required.

Key Features

• Assay Type: Sandwich ELISA
• Detection Range: 0.3 – 10 ng/mL
• Sensitivity: 0.1 ng/mL
• Sample Volume: 10 μL
• Assay Duration: ~2.5 hours
• Storage Conditions: 2–8 °C
• Shelf Life: 6 months
• Precision:
  • Intra-assay CV <10%
  • Inter-assay CV <12%

Assay Principle

The pre-coated 96-well plate captures hs-CRP from samples. A HRP-conjugated anti-hs-CRP antibody is added, forming a sandwich complex. After incubation and washing, TMB substrate is introduced. A blue color develops, turning yellow upon reaction termination. The optical density (OD) is measured at 450 nm, and the hs-CRP concentration is calculated based on a standard curve.

Kit Components

Sample Types & Preparation

• Serum / Plasma: Collect, centrifuge at 2,000–3,000 rpm for 20 minutes.
• Urine / CSF / Body Fluids: Collect and centrifuge; use supernatant.
• Cell Culture Supernatant: Clarify by centrifugation.
• Tissue Homogenate: Homogenize tissue in PBS (pH 7.4), centrifuge at 4 °C.

Note: Avoid sodium azide (NaN₃), which inhibits HRP. Minimize freeze-thaw cycles. Samples can be stored at -20°C.

Assay Procedure Summary

1. Standard Dilution: Prepare six standards (13.5 → 0.75 ng/mL).
2. Add 40 μL sample diluent + 10 μL sample to each well (except blank).
3. Incubate 30 minutes at 37°C, sealed.
4. Wash plate 5× with 1× wash buffer.
5. Add 50 μL HRP-conjugated antibody, incubate 30 min.
6. Wash, then add 50 μL each of Chromogen A and B.
7. Incubate 15 minutes in the dark.
8. Add 50 μL stop solution, and read absorbance at 450 nm within 15 minutes.

Performance & Data Analysis

Generate a log-log standard curve using OD450 readings. Multiply sample results by dilution factor (typically ×5) for final concentration.

The Human hs-CRP ELISA Kit (SL0881Hu) provides a powerful tool for precise quantification of low-level inflammatory responses, enabling researchers to track subtle biomarker fluctuations across various disease states. With high sensitivity, reliable performance, and compatibility with multiple sample types, this kit is ideal for cardiovascular disease research, metabolic disorder monitoring, infection surveillance, and inflammation-related studies in both clinical and academic settings.