Gibco™ Human EGF, Animal-Free Recombinant Protein (PeproTech®)

Gibco™ Human EGF, Animal-Free Recombinant Protein (PeproTech®) is a high-purity recombinant human Epidermal Growth Factor (EGF) produced in E. coli and manufactured under stringent quality standards for research applications. This animal-origin free formulation ensures consistency, safety, and suitability for sensitive cell culture and stem cell workflows.

Human EGF is a potent mitogenic factor that binds to the Epidermal Growth Factor Receptor (EGFR), activating critical downstream signaling pathways that regulate cell proliferation, survival, differentiation, and migration.

Key Features

• Animal-Origin Free (AF)
• Recombinant human protein expressed in E. coli
• ≥98% purity (SDS-PAGE & HPLC)
• Endotoxin level: <0.1 EU/µg
• High biological activity (≥ 1 × 10⁷ units/mg)
• Lyophilized, preservative-free formulation
• Suitable for stem cell and primary cell applications

Biological Activity

• Molecular Weight: 6.2 kDa
• Length: 53 amino acids
• Contains 3 intramolecular disulfide bonds
• ED50 ≤ 0.1 ng/mL (Balb/c 3T3 proliferation assay)

This high specific activity ensures reliable stimulation of cell proliferation and signal transduction pathways.

Mechanism of Action

EGF binds to EGFR (170 kDa receptor tyrosine kinase), inducing receptor dimerization and activation. This triggers multiple downstream pathways, including:

• RAS–RAF–MEK–ERK
• PI3K–AKT
• PLCγ–PKC
• STAT signaling
• NF-κB pathway involvement

EGF plays a key role in tissue growth, epithelial regeneration, stem cell expansion, and oncogenic signaling research.

Applications

• Mammalian cell culture supplementation
• Cell proliferation assays
• Stem cell (PSC/iPSC) expansion
• Cell differentiation and induction
• Cell stimulation studies
• Western blot controls
• ELISA standards
• Bioactivity assays
• Cancer research and EGFR signaling studies

Product Specifications

Available Sizes

Gibco™ Human EGF, Animal-Free Recombinant Protein (PeproTech®) delivers high purity, low endotoxin, and exceptional bioactivity for advanced cell culture and signaling research. Its animal-origin free production ensures reproducibility and compatibility with sensitive stem cell and translational research workflows.