Gibco™ DMEM, powder, low glucose, pyruvate

Gibco™ Dulbecco's Modified Eagle Medium (DMEM) is a versatile and widely used basal medium optimized for the growth of various mammalian cells. This low glucose formulation with sodium pyruvate supports applications where reduced glucose concentrations are essential for physiological relevance or specific metabolic requirements.

Key Features

1. Modified Composition:
   • With Additives:
     • Low Glucose (1 g/L): Suitable for cells requiring reduced glucose levels for metabolic studies or reduced stress conditions.
     • L-Glutamine: Enhances protein synthesis and metabolic activity.
     • Sodium Pyruvate: Provides an additional energy source and supports cellular respiration.
     • Phenol Red: Acts as a pH indicator for monitoring culture conditions.
   • Without Additives:
     • No HEPES: Requires a CO₂ buffering system.
     • No Sodium Bicarbonate: Supplementation required during preparation.
2. Animal-Origin-Free:
   • Manufactured under cGMP compliance and ISO 13485 standards, ensuring consistent quality and suitability for research and manufacturing.
3. Powder Form:
   • Requires supplementation with sodium bicarbonate, pH adjustment, and filtration for final preparation.
4. Shelf Life:
   • Long-term stability with a shelf life of 36 months from the date of manufacture.

Applications

• Cell Culture:
  • Supports a variety of primary cells (e.g., fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells).
  • Suitable for widely used cell lines such as HeLa, 293, Cos-7, and PC-12.
• Metabolic Studies:
  • The low glucose concentration is ideal for studying cellular energy pathways and metabolism under physiological conditions.
• Protein Expression:
  • Supports efficient recombinant protein production in transfected cell lines.
• Stem Cell Research:
  • Provides a controlled environment for culturing stem cells and precursor cells.
• Drug Development:
  • Suitable for applications requiring low-glucose conditions for drug screening or testing cell survival and proliferation.

Preparation Instructions

1. Suspend Powder:
   • Dissolve the powder in distilled water.
   • Add sodium bicarbonate according to protocol for optimal buffering.
2. Adjust pH:
   • Titrate to physiological pH (7.2–7.4) using a pH meter.
3. Sterilize:
   • Filter through a 0.22 μm membrane filter to ensure sterility.
4. Supplementation:
   • Add Fetal Bovine Serum (FBS) at a concentration of 10% or as required for specific applications.
5. Storage:
   • Store prepared media at 2–8°C in a light-protected environment.

Catalog-Specific Details

Advantages of Low Glucose and Sodium Pyruvate

• Low Glucose:
  • Mimics physiological glucose levels, reducing the risk of glucose-induced metabolic alterations.
  • Suitable for cells sensitive to high glucose environments.
• Sodium Pyruvate:
  • Enhances energy production and cell survival under low-glucose conditions.
  • Supports metabolic flexibility by acting as an additional carbon source.

Specifications

This low glucose DMEM formulation is ideal for research applications requiring metabolic control and is widely used in studies involving primary cells, metabolic assays, and low-stress culture conditions.