Gibco™ CD Hybridoma AGT™ Medium, Powder

Gibco™ CD Hybridoma AGT™ Medium is a serum-free, protein-free, and chemically defined medium specifically designed to support the growth of hybridomas and myeloma cell lines used in monoclonal antibody (mAb) production. Supplied in a dry, granulated format using Advanced Granulation Technology™ (AGT™), this medium offers convenient, fast, and reproducible preparation with rapid solubility and pH stability.

This formulation is animal origin–free (AOF) and does not contain L-glutamine or phenol red, providing flexibility and stability for users who prefer to supplement with GlutaMAX™ or L-glutamine based on specific process needs. It includes sodium pyruvate and sodium bicarbonate to support optimal cell metabolism and buffering.

Manufactured under cGMP-compliant and ISO 13485–certified conditions in an FDA-registered facility, this medium is available for research and further manufacturing use and is backed by a Type II Drug Master File (DMF) for FDA-regulated submissions.

Key Features

• Chemically Defined and Protein-Free: Ideal for antibody purification and regulatory compliance.
• Animal Origin-Free (AOF): Minimizes risk of contamination and variability.
• AGT™ Powder Format: Granulated dry media dissolves rapidly and pH adjusts instantly—reduces prep time.
• Supports Suspension & Adherent Cultures: Promotes high-density growth and IgG production in hybridomas and recombinant myelomas.
• No Glutamine Added: Customize with 8 mM L-glutamine or GlutaMAX™ as needed.
• Regulatory Support: FDA Type II DMF available upon request for submission purposes.

Applications

• Hybridoma and Myeloma Cell Culture:
  • Supports monoclonal antibody production workflows in research and preclinical biomanufacturing.
• GMP-Ready Cell Expansion:
  • Chemically defined formulation suitable for scale-up and further manufacturing.
• Antibody Purification Workflows:
  • Low protein and serum-free composition facilitates downstream purification.
• Cholesterol-Dependent Cell Culture:
  • NS0 and similar cell lines can be supported with the addition of 250× Cholesterol Lipid Concentrate.

Specifications

Gibco™ CD Hybridoma AGT™ Medium provides a scalable, high-performance, and regulatory-friendly solution for antibody production. Its chemically defined, animal-origin-free formulation and convenient granulated powder format make it ideal for bioprocessing workflows, enabling efficient preparation and consistent hybridoma performance.