Gibco™ Anti-Clumping Agent

Gibco™ Anti-Clumping Agent is a concentrated, animal origin-free, and chemically defined liquid reagent designed to reduce cell clumping in suspension cultures. By improving cell suspension and viability, it enhances overall culture conditions, leading to higher cell densities and improved protein expression in mammalian and insect cell lines.

Key Features:

1. Reduces Cell Clumping:
   • Prevents aggregation in suspension cultures, improving cell dispersion and promoting consistent growth.
2. Animal Origin-Free and Chemically Defined:
   • Free of animal-derived components, proteins, enzymes, and hydrolysates, ensuring safety and consistency.
3. Improves Cell Viability and Protein Yield:
   • Higher viable cell densities stabilize culture conditions, leading to better protein production.
4. Gentle on Cells:
   • Offers a non-invasive solution to cell clumping compared to harsh enzymatic dissociation agents.
5. Convenient Packaging:
   • Available in 20 mL and 100 mL sizes, with options for custom packaging.

Specifications:

Applications:

1. Suspension Cell Culture:
   • Ideal for maintaining and stabilizing suspension cultures of mammalian and insect cells.
2. Protein Expression Studies:
   • Improves culture conditions for enhanced protein yield in research and production workflows.
3. High-Density Cell Cultures:
   • Supports scaling up cultures for biomanufacturing or research purposes.
4. Post-Transfection Stabilization:
   • Recommended for use after transfection to maintain suspension stability.
5. Non-Invasive Aggregation Prevention:
   • Provides a gentle alternative to harsh enzymatic cell dissociation reagents.

Advantages:

1. Consistent and Reliable:
   • Chemically defined components ensure batch-to-batch consistency and reproducible results.
2. Regulatory-Friendly:
   • Free of animal-origin components, minimizing the risk of contamination from adventitious agents.
3. Easy Integration:
   • Simple titration-based usage integrates seamlessly into existing suspension culture protocols.
4. Customizable Packaging:
   • Options for larger volumes available to meet high-throughput lab needs.
5. Safe for Sensitive Applications:
   • Avoids harsh enzymatic reactions, preserving cell health and viability.

Protocol Overview:

1. Prepare Media:
   • Add Anti-Clumping Agent to culture media at a 1:100 to 1:1000 dilution, depending on the extent of clumping.
2. Mix Thoroughly:
   • Ensure the reagent is evenly distributed throughout the media.
3. Add Cells:
   • Introduce cells into the media with Anti-Clumping Agent.
4. Monitor Cultures:
   • Observe cultures for reduced aggregation and improved growth.
5. Post-Transfection Use:
   • If used in transfection workflows, add only after the transfection process to avoid interference.

Ordering Information:

Storage and Handling:

• Storage Conditions: Store at 2°C to 8°C.
• Shelf Life: Refer to product label for expiration details.
• Shipping Conditions: Room temperature.

Related Products:

• Gibco™ TrypLE™ Express Enzyme: For enzymatic cell dissociation.
• Gibco™ CD CHO Medium: For culturing CHO cells in suspension.
• Gibco™ Insect Cell Medium: Optimized for insect cell cultures.

Product Use and Limitations:

• Intended Use: For research use only. Not for use in diagnostic or therapeutic procedures.
• Limitations: Avoid use during transfection processes as it may interfere with lipid cationic transfection reagents.

The Gibco™ Anti-Clumping Agent is a versatile and effective solution for maintaining stable suspension cultures. Its animal origin-free and chemically defined formulation ensures reproducibility, safety, and compatibility with advanced research and production workflows.