Gibco™ Amphotericin B, 0.25 to 2.5 μg/mL

Broad-Spectrum Antifungal Agent for Preventing Yeast & Fungal Contamination in Cell Cultures

Gibco™ Amphotericin B is a broad-spectrum antifungal agent derived from Streptomyces nodosus, commonly used to prevent and control fungal contamination in cell culture systems. It works by binding to sterols in fungal membranes, leading to increased permeability and pore formation, ultimately disrupting fungal growth.

This formulation contains 250 μg/mL of Amphotericin B with 205 μg/mL of sodium deoxycholate as a solubilizing agent, making it highly effective for use in mammalian, bacterial, and fungal cell cultures.

Key Features & Benefits

✅ Broad-Spectrum Antifungal Activity – Prevents contamination by yeasts and multicellular fungi

✅ Sterile, Ready-to-Use Solution – No additional preparation required

✅ Recommended Working Concentration (0.25 – 2.5 μg/mL) – Ensures effective fungal protection without cytotoxicity

✅ Compatible with Various Cell Culture Systems – Suitable for mammalian, bacterial, and fungal cultures

✅ Prevents Loss of Valuable Cultures – Reduces experimental failures due to fungal contamination

✅ Contains Sodium Deoxycholate – Enhances solubility and stability

Applications

🦠 Prevention of Fungal Contamination – Protects cell cultures from yeast and filamentous fungi

🔬 Mammalian & Stem Cell Cultures – Ensures sterile conditions for sensitive cell lines

🧫 Microbial Research & Bacterial Cultures – Maintains uncontaminated bacterial growth conditions

⚗️ Biopharmaceutical & Drug Testing – Prevents fungal interference in bioproduction

🛠 Diagnostic & Clinical Laboratories – Used in cell-based assays and microbial detection

Technical Specifications

Recommended Usage Guidelines

1️⃣ Dilute Gibco™ Amphotericin B to the required working concentration (0.25 – 2.5 μg/mL) in the desired culture medium.

2️⃣ Gently mix to ensure even distribution before adding to cell cultures.

3️⃣ Use in combination with other antibiotics for broader contamination control (if needed).

4️⃣ Store at –20°C to maintain long-term stability and prevent degradation.

⚠ Do not use at concentrations exceeding 2.5 μg/mL to avoid cytotoxic effects on sensitive mammalian cell lines.

Regulatory & Safety Information

⚠️ For Research Use Only. Not for human or veterinary therapeutic applications.

⚠️ Highly Potent Antifungal Agent – Handle with appropriate safety precautions in biosafety level (BSL-2) laboratories.

• Proper PPE (gloves, lab coat, and eye protection) is required.
• Dispose of waste according to local hazardous material regulations.

Why Choose Gibco™ Amphotericin B?

🔹 Trusted Antifungal Protection – Reliable against fungal and yeast contamination

🔹 Standardized & High Purity – Ensures consistent performance across multiple experiments

🔹 Optimized for Cell Culture – Minimizes cytotoxicity while maintaining antifungal efficacy

🔹 Ready-to-Use Liquid Formulation – Saves time compared to powder-based formulations

For bulk orders, technical support, or additional information, contact LabMart Limited today.