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Thermo Scientific™ FastDigest™ KflI, 20 μL (20 Reactions)
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ FastDigest KflI is a rapid-acting restriction enzyme that recognizes the sequence 5′ GG^GWCCC 3′ / 3′ CCCWGG^G 5′, producing 5′ protruding ends. Optimized for use at 37 °C, it ensures complete digestion within 5–15 minutes using the universal FastDigest Buffer system.
As part of the FastDigest™ enzyme line, KflI is fully active in both the FastDigest and FastDigest Green Buffers, enabling single, double, or multiple digestions in one step without buffer changes. Its optimized formulation eliminates star activity and supports direct downstream workflows with DNA modifying enzymes, including T4 DNA Ligase, Klenow Fragment, T4 DNA Polymerase, and alkaline phosphatases.
Key Features
- Rapid digestion – complete DNA cleavage in 5–15 minutes at 37 °C.
- Universal buffer system – 100% enzyme activity in both FastDigest and FastDigest Green Buffers.
- Direct loading – FastDigest Green Buffer includes density reagent and tracking dyes for electrophoresis.
- No star activity – optimized composition prevents nonspecific cleavage.
- Streamlined workflow – downstream enzymes are compatible within the same buffer.
- Broad enzyme portfolio – part of a collection of 176 FastDigest enzymes.
Applications
- Molecular cloning.
- Restriction site mapping.
- Genotyping and SNP analysis.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP).
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | KflI |
| Recognition Sequence | 5′ GG^GWCCC 3′ / 3′ CCCWGG^G 5′ |
| Cleavage Product | 5′ protruding ends |
| Isoschizomers | SanDI, Sse1825I |
| Optimal Reaction Temperature | 37 °C |
| Digestion Time | 5–15 minutes |
| Compatible Buffers | 10X FastDigest Buffer, 10X FastDigest Green Buffer |
| Heat Inactivation | No |
| Methylation Sensitivity | CpG methylation-sensitive; not Dam/Dcm-sensitive |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Formats
| Catalog Number | Quantity | Reactions | Format |
|---|---|---|---|
| FD2164 | 20 µL | ~20 reactions | Enzyme + Buffers |
Contents:
- FastDigest KflI enzyme (20 µL)
- 10X FastDigest Buffer (1.0 mL)
- 10X FastDigest Green Buffer (1.0 mL)
Thermo Scientific™ FastDigest KflI offers laboratories a reliable, fast, and high-fidelity restriction enzyme for cloning and molecular biology workflows. Its universal buffer system, rapid digestion performance, and compatibility with downstream enzymatic steps eliminate workflow bottlenecks and ensure reproducibility. With its ability to perform complete digestions in minutes, it is a valuable enzyme for modern genetic research and analysis.