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Thermo Scientific™ FastDigest™ RsaI, 100 μL (100 Reactions)
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes and Substrates | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Rapid, high-purity restriction enzyme for GT^AC DNA cleavage in 5–15 minutes
The Thermo Scientific™ FastDigest RsaI restriction endonuclease recognizes the sequence GT^AC and cleaves between the T and A nucleotides, producing blunt ends. Optimized for use at 37°C in the universal FastDigest Buffer, this enzyme allows complete digestion within 5–15 minutes without the need for buffer changes or DNA clean-up steps.
Part of the FastDigest enzyme range, RsaI delivers 100% activity in both FastDigest and FastDigest Green Buffers, enabling rapid single, double, or multiple digestions. These buffers are fully compatible with downstream enzymes, allowing direct addition of DNA modifying enzymes such as T4 DNA Ligase, Klenow Fragment, and alkaline phosphatase without purification.
Isoschizomers: AfaI, CviQI, RsaNI.
Key Features & Benefits
- Ultra-Fast Digestion – Complete DNA digestion in 5–15 minutes.
- Universal Buffer Compatibility – All FastDigest enzymes perform in the same buffer.
- Direct Gel Loading – FastDigest Green Buffer includes density reagent and tracking dyes.
- Downstream Ready – 100% compatible with ligases, polymerases, and phosphatases.
- No Star Activity – Optimized buffer composition prevents non-specific cutting.
- Convenient Multiplexing – Perform multiple digestions without intermediate purification.
Applications
- Molecular Cloning – Efficient generation of DNA fragments for cloning workflows.
- Restriction Site Mapping – Rapid identification of restriction patterns.
- Genotyping – Fast genetic variant analysis.
- Southern Blotting – Preparation of DNA for probe hybridization.
- RFLP Studies – Detecting genetic polymorphisms.
- SNP Analysis – High-precision variant detection.
Recognition Sequence & Cleavage Pattern
| Strand | Sequence |
|---|---|
| 5′ | G T ↓ A C |
| 3′ | C A ↑ T G |
Technical Specifications
| Property | Details |
|---|---|
| Enzyme | RsaI |
| Recognition Site | GT^AC |
| Overhang Type | Blunt |
| Quantity | 100 μL (100 reactions) |
| Compatible Buffers | 10X FastDigest Buffer / 10X FastDigest Green Buffer |
| Optimal Temperature | 37°C |
| Methylation Sensitivity | CpG Methylation-Sensitive; Not Dam or Dcm Methylation-Sensitive |
| Heat Inactivation | No |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional Cloning |
| Storage | –20°C |
| Formulation | Supplied with both FastDigest and FastDigest Green Buffers |
Kit Contents
- 100 μL FastDigest RsaI enzyme
- 1.0 mL 10X FastDigest Buffer
- 1.0 mL 10X FastDigest Green Buffer
Ordering Information
| Catalog Number | Quantity |
|---|---|
| FD1124 | 100 μL (100 reactions) |
The Thermo Scientific™ FastDigest RsaI combines high specificity with exceptional speed, allowing researchers to perform complete DNA digestion in minutes without compromising accuracy. Its universal buffer compatibility streamlines workflows, supports seamless integration with downstream applications, and reduces processing time, making it the preferred choice for time-sensitive cloning and molecular analysis projects.