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Thermo Scientific™ FastDigest™ SalI, 200 μL (200 Reactions)
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ FastDigest SalI is a high-performance restriction enzyme that recognizes the sequence 5' G^TCGAC 3' / 3' CAGCT^G 5' and cleaves to generate 5′ overhangs. Designed for rapid and efficient digestion, FastDigest SalI achieves complete DNA cleavage within 5–15 minutes at 37 °C, offering superior speed and reliability compared to conventional restriction enzymes.
As part of the FastDigest™ enzyme line, it is fully optimized for use with the universal FastDigest and FastDigest Green Buffers, ensuring 100% activity for all enzymes in this system. This enables single, double, or multiple digestions without buffer changes and eliminates additional DNA clean-up steps.
Key Features
- Fast and efficient – complete digestion in 5–15 minutes at 37 °C.
- Universal compatibility – 100% activity of all FastDigest enzymes in both FastDigest and FastDigest Green Buffers.
- No star activity – optimized buffer composition prevents non-specific cleavage.
- Streamlined workflow – downstream enzymes (T4 DNA ligase, Klenow fragment, T4 DNA polymerase, alkaline phosphatase) work directly in FastDigest Buffer.
- Direct gel loading – FastDigest Green Buffer includes tracking dyes and density reagent for convenient electrophoresis.
- Wide range availability – part of a comprehensive portfolio of 176 FastDigest enzymes.
Applications
- Molecular cloning workflows.
- Restriction site mapping.
- Genotyping and SNP analysis.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP) studies.
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | SalI |
| Recognition Sequence | 5' G^TCGAC 3' / 3' CAGCT^G 5' |
| Cleavage Product | 5′ protruding ends |
| Optimal Reaction Temperature | 37 °C |
| Complete Digestion Time | 5–15 minutes |
| Inactivation Temperature | 65 °C |
| Methylation Sensitivity | Sensitive to CpG methylation; not Dam/Dcm methylation-sensitive |
| Compatible Buffers | 10X FastDigest Buffer, 10X FastDigest Green Buffer |
| Storage Temperature | –20 °C |
| Research Category | Traditional cloning |
| Type IIS Restriction Enzyme | No |
Available Format
| Catalog Number | Quantity | Format | No. of Reactions |
|---|---|---|---|
| FD0644 | 200 µL | Includes FastDigest SalI, 10X FastDigest Buffer (1 mL), 10X FastDigest Green Buffer (1 mL) | ~200 reactions |
Thermo Scientific™ FastDigest SalI provides researchers with a rapid, reliable, and buffer-universal restriction enzyme solution optimized for molecular cloning and DNA analysis. With complete digestion in under 15 minutes, compatibility with downstream enzymatic steps, and elimination of star activity, it simplifies workflows while maintaining precision and reproducibility. This makes it a preferred choice for modern cloning and genotyping applications requiring high efficiency and flexibility.