Invitrogen™ TGF beta 1 Human ProcartaPlex™ Simplex Kit

The Human TGF-beta 1 Simplex ProcartaPlex Kit is a robust and efficient tool for the quantification of Transforming Growth Factor beta 1 (TGF-beta 1) protein levels in biological samples. Leveraging Luminex xMAP technology, this kit provides high-sensitivity and high-throughput analysis with minimal sample volume requirements.

Key Features and Benefits

1. High Sensitivity and Specificity:
   • Measures TGF-beta 1 protein with high sensitivity.
   • Utilizes two matched antibodies for specific binding to distinct epitopes.
2. Luminex xMAP Technology:
   • Allows simultaneous detection and quantification of up to 80 protein targets in a single sample.
   • Uses spectrally unique beads for multiplexing, reducing time and sample volume compared to ELISA.
3. Minimal Sample Volume:
   • Requires as little as 25 µL of plasma or serum or 50 µL of cell culture supernatant.
4. Fast and Efficient:
   • Results can be obtained in as little as 4 hours.
5. Comprehensive Assay Range:
   • Lot-specific assay range: 1.1–4500 pg/mL.
6. Species Compatibility:
   • Designed specifically for human samples.
7. Multiplexing Capability:
   • Combines up to 80 protein targets in a single well when used with other ProcartaPlex kits (except with TGF-beta 1, which is not combinable).

Specifications

Kit Components

• 1 vial Capture Beads (50X): TGF-beta 1-specific beads.
• 1 vial Biotinylated Detection Antibody (50X): Detects bound TGF-beta 1.
• 2 vials Human Standard TGF-beta 1 (lyophilized): For assay standardization.

Applications

1. Protein Quantification:
   • Accurate measurement of TGF-beta 1 in serum, plasma, and cell culture supernatants.
2. Immunology Research:
   • Studying the role of TGF-beta 1 in immune modulation and disease.
3. Disease Biomarker Analysis:
   • Assessment of TGF-beta 1 in inflammatory diseases, cancer, and fibrosis research.
4. High-Throughput Screening:
   • Multiplexing capabilities for large-scale studies.

Advantages over Traditional ELISA

• Efficiency: Simultaneous measurement of multiple proteins in a single sample.
• Speed: Results in 4 hours, compared to longer ELISA protocols.
• Reduced Sample Volume: Requires only a fraction of the volume needed for ELISA.
• Precision: Uses Luminex’s dual-laser detection for high accuracy.

Usage Notes

• Sample Activation Required: TGF-beta 1 measurement requires specific sample activation (refer to the instruction manual).
• Non-Combinable: It is not recommended to combine the TGF-beta 1 Simplex Kit with other ProcartaPlex Simplex kits.
• Basic Kit Requirement: Requires the ProcartaPlex Human Basic Kit (Cat. No. EPX010-10420-901) for operation.

Storage and Handling

• Store all kit components at 2°C to 8°C.
• Follow proper laboratory protocols for handling and disposal.

For Research Use Only

This kit is intended for research purposes and is not suitable for diagnostic or clinical procedures.