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Invitrogen™ Galactose Colorimetric Detection Kit, 2 x 96 Tests
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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | -20°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Galactose Colorimetric Detection Kit is a complete, ready-to-use assay for the quantitative measurement of galactose in serum, plasma, buffers, and tissue culture media. Utilizing a robust colorimetric format with detection at 560 nm, the kit provides high sensitivity (0.493 mg/dL) and a broad standard curve range (0.78–25 mg/dL) for accurate galactose quantification.
Assay Principle
The assay measures galactose based on galactose oxidase-mediated oxidation:
- Galactose oxidase reacts with D-(+)-galactose to generate hydrogen peroxide (H₂O₂).
- In the presence of horseradish peroxidase (HRP), H₂O₂ reacts with a proprietary substrate to produce a pink-colored product.
- The absorbance is read at 560 nm using a microplate reader, with color intensity proportional to galactose concentration.
Key Features
- High Sensitivity – Detects as low as 0.493 mg/dL galactose.
- Broad Quantification Range – 0.78–25 mg/dL standard curve.
- Ready-to-Use Kit – Includes plates, standards, buffers, and all required reagents.
- Species Independent – Works across multiple biological sources.
- Simple Workflow – Room temperature 30-minute incubation with direct readout.
Applications
- Metabolic Studies – Assess galactose metabolism in research models.
- Food & Nutrition Research – Measure galactose in dairy, fruit, and vegetable samples (research use only).
- Enzyme Activity Studies – Monitor galactose oxidase-dependent reactions.
- Cell Culture Monitoring – Evaluate sugar metabolism in tissue culture systems.
- Biochemical Pathway Analysis – Study the Leloir pathway and related disorders.
Technical Specifications
| Parameter | Specification |
|---|---|
| Detection Method | Colorimetric (560 nm) |
| Sensitivity | 0.493 mg/dL |
| Standard Curve Range | 0.78–25 mg/dL |
| Sample Types | Serum, Plasma, Buffers, Tissue Culture Media |
| Incubation Time | 30 minutes |
| Format | 96-well microplate |
| Storage | –20°C |
| Shipping | Wet or Dry Ice |
| Unit Size | 2 × 96 Tests |
Kit Contents
- 96-well microplates
- D-(+)-Galactose standard
- Assay buffer
- Galactose oxidase
- Horseradish peroxidase (HRP)
- Substrate solution
- Lot-specific technical datasheet
The Invitrogen™ Galactose Colorimetric Detection Kit is a reliable and precise solution for galactose measurement in biological and biochemical research applications. With its ready-to-use format, high sensitivity, and broad detection range, it is well-suited for metabolic research, enzyme assays, and nutritional studies.