Invitrogen™ Human Ferritin ELISA Kit

The Invitrogen™ Human Ferritin ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of Ferritin levels in human serum, plasma, or cell culture medium. This kit exclusively recognizes both natural and recombinant human Ferritin and is essential for research into iron homeostasis, metabolic regulation, and related disorders.

Principle of the Method

The assay employs a solid-phase sandwich ELISA technique to measure Ferritin levels:

1. Pre-Coated Plate:
   • Microplate wells are pre-coated with a capture antibody specific to Ferritin.
2. Sample and Standard Addition:
   • Samples, standards, or controls are added to the wells and bind to the immobilized capture antibody.
3. Detection Antibody Addition:
   • A biotinylated detection antibody specific to Ferritin is added to form a sandwich complex.
4. Signal Development:
   • Streptavidin-HRP and a substrate solution are added, producing a measurable colorimetric signal proportional to the Ferritin concentration.
5. Measurement:
   • The intensity of the color is measured at 450 nm using a colorimetric microplate reader.

Key Features

1. Rigorous Validation:
   • Each lot is quality-tested for sensitivity, specificity, precision, and consistency.
2. High Sensitivity:
   • Analytical sensitivity of 600 pg/mL.
3. Wide Assay Range:
   • Dynamic range of 0.81-200 ng/mL.
4. Precision:
   • Interassay CV: <12%, Intraassay CV: <10%.
5. Ease of Use:
   • Hands-on time of 1 hr 20 min, with time-to-result of 4 hr 45 min.

Applications

• Iron Homeostasis Research:
  • Study Ferritin's role in iron storage and regulation.
• Metabolic Disorders:
  • Investigate Ferritin levels in metabolic syndromes and iron-related diseases.
• Neurodegenerative Disease Research:
  • Explore Ferritin’s involvement in neurodegenerative conditions and hyperferritinemia-cataract syndrome.
• Inflammatory and Immune Studies:
  • Assess Ferritin as a biomarker for inflammatory and immune responses.
• Therapeutic Development:
  • Evaluate Ferritin as a target in treatments for iron dysregulation and associated disorders.

Specifications

• Analytical Sensitivity: 600 pg/mL
• Assay Range: 0.81-200 ng/mL
• Sample Type/Volume:
  • Plasma: 50 µL
  • Serum: 50 µL
  • Supernatant: 100 µL
• Hands-On Time: 1 hr 20 min
• Time-to-Result: 4 hr 45 min
• Instrument: Colorimetric Microplate Reader
• Interassay CV: <12%
• Intraassay CV: <10%
• Product Size: 96 Tests

Kit Contents

• Pre-coated 96-well plate
• Ferritin Standard
• Assay Diluent concentrate
• Biotinylated Detection Antibody
• SAV-HRP (Streptavidin-HRP)
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers

Target Information

Ferritin:

• Function:
  • Major intracellular iron storage protein regulating soluble and non-toxic iron storage.
  • Stores up to 4,500 atoms of ferric iron in a nontoxic state.
• Structure:
  • Composed of 24 subunits (H and L chains) with a molecular weight of ~450 kDa.
• Role in Health and Disease:
  • Elevated Ferritin levels are associated with inflammation, infection, and iron overload disorders.
  • Ferritin gene mutations are linked to neurodegenerative diseases and hyperferritinemia-cataract syndrome.
• Biological Importance:
  • Plays a critical role in iron homeostasis and innate immunity.

Storage and Stability

• Storage Conditions: Store all components at 2–8°C.
• Shipping Conditions: Wet ice.
• Stability: Proper storage ensures reagent integrity and assay reliability.

The Invitrogen™ Human Ferritin ELISA Kit is an indispensable tool for researchers studying iron metabolism, inflammatory responses, and related disorders. Its rigorous validation, high sensitivity, and ease of use make it ideal for clinical and experimental applications.