Thermo Scientific™ DH5α Competent Cells, 10 x 100 µL

Thermo Scientific DH5α Competent Cells are chemically competent E. coli cells designed for high-efficiency transformation. These cells are widely used in molecular biology applications, including DNA cloning, cDNA library construction, and blue/white colony screening. The genetic modifications in DH5α cells improve plasmid stability and the quality of extracted DNA, making them ideal for routine and high-throughput cloning workflows.

Key Features:

1. High Transformation Efficiency:
   • Transformation efficiency of >1×10⁹ cfu/µg pUC19 DNA, ensuring excellent cloning success rates.
2. Genetic Modifications:
   • φ80dlacZΔM15 marker: Allows blue/white colony screening on media with X-Gal or Bluo-Gal.
   • recA1 mutation: Enhances stability of cloned DNA by reducing recombination.
   • endA1 mutation: Improves quality of extracted plasmid DNA by reducing endonuclease activity.
3. Convenient Packaging:
   • 10 vials (100 µL per vial), each suitable for two transformation reactions.
   • Includes S.O.C. Outgrowth Medium for post-transformation recovery.
4. Versatile Applications:
   • Ideal for constructing gene banks or cDNA libraries.
   • Suitable for site-directed mutagenesis and routine cloning of PCR products.

Applications:

1. DNA Cloning:
   • High-efficiency transformation for cloning plasmids and PCR products.
2. Blue/White Colony Screening:
   • Enables visual selection of successful transformants.
3. Library Construction:
   • Efficient generation of cDNA libraries using plasmid vectors.
4. Site-Directed Mutagenesis:
   • Supports targeted DNA modifications.

Genotype:

• F– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ– thi–1 gyrA96 relA1

Specifications:

Kit Contents:

• 10 x 100 µL DH5α Competent Cells
• pUC19 DNA (50 µL) at 10 pg/µL for transformation controls.
• S.O.C. Medium (5 mL) for post-transformation cell recovery.

Advantages:

1. High Transformation Rates:
   • Ensures successful cloning of plasmid DNA with minimal effort.
2. Improved Plasmid Stability:
   • Reduced recombination and endonuclease activity enhance DNA integrity.
3. Ease of Use:
   • Pre-aliquoted vials streamline experimental workflows.

Storage and Handling:

• Store at -80°C to maintain cell viability.
• Handle with care to avoid repeated freeze-thaw cycles.