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Sigma-Aldrich DirectLoad™ PCR 100 bp Low Ladder
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ready-to-use marker for DNA electrophoresis
Specifications:
General description
Sigma′s DirectLoad™ PCR 100 bp Low Ladder contains 10 dsDNA fragments from 100 − 1,000 bases, evenly spaced in 100 bp repeats. Ladder should be loaded in a single lane on an agarose gel.
Application
Suitable for size determination of PCR generated DNA fragments with either agarose or polyacrylamide gels.
Features and Benefits
- Ready-to-load
- Easy-to-use
- Popular band sizes
Components
Sigma′s DirectLoad™ PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 2.0%.