Thermo Scientific™ Oxoid™ Lauryl Tryptose Broth w/MUG (Dehydrated)

Thermo Scientific™ Oxoid™ Lauryl Tryptose Broth w/MUG is a selective and differential medium for the detection and presumptive identification of Escherichia coli from food, water, dairy, and environmental samples. It incorporates MUG (4-methylumbelliferyl-ß-D-glucuronide), which is cleaved by β-glucuronidase (GUD), an enzyme specific to E. coli, resulting in a fluorescent end-product that is easily detectable under UV light.

Key Features

1. Selectivity

• Inhibits Gram-positive bacteria, particularly bacilli and fecal streptococci, ensuring selectivity for Escherichia coli and coliform organisms.

2. Fluorescent Identification

• The addition of MUG provides a fluorescent readout for rapid and presumptive confirmation of E. coli.

3. Versatile Applications

• Recommended for:
  • Water and environmental testing.
  • Dairy product and food safety analysis.
  • Meat and shellfish testing per FDA/BAM guidelines.

4. Reliable Results

• Produces dependable results within 24–48 hours, ensuring timely detection of fecal contamination.

5. Regulatory Compliance

• Compliant with:
  • FDA/BAM methods.
  • AFNOR, BSI, and ISO standards.

Applications

1. Water Safety Monitoring:
   • Detect and differentiate Escherichia coli and coliforms in drinking and recreational water.
2. Food Safety Testing:
   • Analyze dairy, meat, shellfish, and processed foods for contamination.
3. Environmental Monitoring:
   • Identify fecal contamination in wastewater, sewage, and agricultural runoff.
4. Clinical Diagnostics:
   • Screen for E. coli in public health investigations.
5. Veterinary Microbiology:
   • Assess water and feed quality for animal health.

Composition (Typical Formula)

Preparation Instructions

1. Suspend 35.7g of dehydrated medium in 1 litre of distilled water.
2. Heat gently to dissolve completely.
3. Dispense into suitable containers and sterilize by autoclaving at 121°C for 15 minutes.
4. Allow the medium to cool before use.

Advantages

1. Enhanced Selectivity and Specificity:
   • Designed to suppress non-target organisms while allowing E. coli and coliform growth.
2. Rapid Detection:
   • Fluorescent MUG-based identification speeds up confirmatory testing.
3. Wide Range of Applications:
   • Suitable for food, water, and environmental testing.
4. Ease of Use:
   • Gas production and fluorescence provide straightforward, reliable results.

Quality Control

Specifications

• Form: Powder
• Yield: 500g makes approximately 14.0L of medium
• Certifications/Compliance: AFNOR, BSI, ISO, FDA/BAM
• Unit Size: Each

Storage and Shelf Life

1. Store the dehydrated medium at 10–30°C in a tightly sealed container.
2. Use before the expiration date printed on the label.

Why Choose Thermo Scientific™ Oxoid™ Lauryl Tryptose Broth w/MUG?

Thermo Scientific™ Oxoid™ Lauryl Tryptose Broth w/MUG is a reliable, industry-standard medium for detecting Escherichia coli and coliforms in various matrices. Its MUG fluorescence technology ensures rapid, accurate identification, making it an essential tool for laboratories focused on microbiological testing in food safety, water quality, and environmental health.