Thermo Scientific™ Oxoid™ Endo Agar

The Thermo Scientific™ Oxoid™ Endo Agar (Dehydrated) is a selective and differential medium designed for the isolation and differentiation of Enterobacteriaceae, particularly coliforms, from potable water, dairy products, and foods. This medium, when used with Basic Fuchsin (BR0050), distinguishes lactose-fermenting from non-lactose-fermenting organisms, aiding in the detection of foodborne and waterborne contaminants.

Key Features

1. Selective and Differential:
   • Distinguishes lactose-fermenting coliforms (Escherichia coli) from non-lactose fermenters based on colony color and medium interaction.
2. Indicator Groups:
   • Provides a reliable measure of food quality, spoilage potential, and water safety by detecting coliform organisms.
3. Lactose Fermentation Detection:
   • Lactose fermenters produce deep red colonies with a golden metallic sheen and color the surrounding medium.
   • Non-lactose fermenters form colorless translucent colonies against a pink to colorless medium.
4. Wide Application:
   • Suitable for testing potable water, wastewater, dairy products, and foods for coliform presence.
5. APHA-Recommended:
   • Endorsed by the American Public Health Association (APHA) for water and food microbiology testing.

Applications

1. Water Microbiology

• Detection and enumeration of coliform organisms in potable water and wastewater.

2. Food Microbiology

• Isolation of Enterobacteriaceae and coliforms in dairy products and processed foods.
• Monitoring food safety and spoilage indicators in food production environments.

3. Environmental Monitoring

• Testing for coliform contamination in environmental samples such as soil and water.

Specifications

Preparation Instructions

1. Suspend: Suspend 44.5g of Endo Agar Base in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation until fully dissolved. Do not autoclave.
3. Cool and Add Basic Fuchsin: Cool to 45–50°C and aseptically add Basic Fuchsin (BR0050) as per the manufacturer's instructions.
4. Dispense: Pour into sterile Petri dishes and allow to solidify.

Advantages

1. Clear Differentiation: Easily distinguishes lactose-fermenting from non-lactose-fermenting organisms.
2. Broad Utility: Suitable for food safety, water quality testing, and environmental monitoring.
3. Reliable Performance: Consistently detects Enterobacteriaceae, including coliforms, with high sensitivity.
4. Economical: High yield of 13.9L per 500g, ideal for routine laboratory use.

Diagnostic Features

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C in a dry environment.
• Shelf Life: Refer to product label for expiration date.

Packaging

• Product: Endo Agar Base (Dehydrated)
• Quantity: 500g
• Yield: 13.9L of prepared medium

The Thermo Scientific™ Oxoid™ Endo Agar (Dehydrated) is a reliable and efficient medium for detecting and differentiating Enterobacteriaceae and coliform organisms in food, water, and environmental samples. Its compliance with APHA guidelines, ease of interpretation, and suitability for diverse microbiological applications make it indispensable for microbiology laboratories focused on food safety, water quality, and environmental monitoring.