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Thermo Scientific™ Oxoid™ Mannitol Salt Agar (Dehydrated), 500G
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Formula | For 4.5L medium | ||
The Thermo Scientific™ Oxoid™ Mannitol Salt Agar (Dehydrated) is a selective and differential medium used for the presumptive detection, isolation, and enumeration of pathogenic staphylococci, including Staphylococcus aureus, in samples such as milk, food, fish, and other specimens. The high salt concentration inhibits most bacteria, while allowing halophilic organisms, including staphylococci, to grow.
Key Features
- Highly Selective:
- The high salt concentration (7.5%) inhibits non-staphylococcal organisms, allowing selective growth of Staphylococcus spp.
- Differentiates Staphylococci:
- Staphylococcus aureus: Produces yellow colonies surrounded by yellow halos due to mannitol fermentation.
- Staphylococcus epidermidis: Produces pink colonies, with the medium turning pink.
- Reliable Performance:
- Provides clear and easy differentiation of pathogenic and non-pathogenic staphylococci.
- Versatile Applications:
- Ideal for microbiological testing in milk, food, fish, and other specimens.
Applications
1. Food Microbiology
- Detection of Staphylococcus aureus in dairy products, processed foods, and seafood.
2. Clinical Microbiology
- Isolation and differentiation of Staphylococcus spp. from clinical specimens.
3. Environmental Microbiology
- Monitoring of staphylococcal contamination in environmental and industrial samples.
4. Research Microbiology
- Studies on halophilic and staphylococcal species differentiation.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Agar |
| Form | Powder |
| Description | Mannitol Salt Agar |
| Applications | Food, clinical, environmental, and research microbiology |
| Quantity | 500g |
| Yield | Varies; refer to product label for details |
Preparation Instructions
- Suspend: Suspend 111g of Mannitol Salt Agar powder in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Dispense: Pour into sterile Petri dishes and allow to solidify.
Advantages
- Selective and Differential: Distinguishes Staphylococcus aureus from other staphylococcal species while inhibiting most other bacteria.
- Reproducible Results: Delivers consistent and reliable differentiation of pathogenic staphylococci.
- Broad Utility: Suitable for microbiological testing across food, clinical, and environmental workflows.
- Cost-Effective: Ideal for routine laboratory testing and high-throughput environments.
Quality Control
| Control | Expected Results |
|---|---|
| Positive Control | Staphylococcus aureus – Yellow colonies with yellow halo |
| Negative Control | Staphylococcus epidermidis – Pink colonies with medium turning pink |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to the product label for expiration date.
Packaging
- Product: Mannitol Salt Agar (Dehydrated)
- Quantity: 500g
The Thermo Scientific™ Oxoid™ Mannitol Salt Agar (Dehydrated) is a reliable and efficient medium for the detection and differentiation of Staphylococcus spp., particularly Staphylococcus aureus. Its selectivity and ease of differentiation make it indispensable for microbiology laboratories focused on food safety, clinical diagnostics, and environmental monitoring.