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Specifications:
| Application | ELISA-Based Assays |
| Storage Temperature | 2-8°C |
| Product Type | Elisa Kit |
| Product Grade | Molecular Biology |
The Interleukin (IL) ELISA Kits are highly sensitive and specific assays designed to detect and quantify various interleukins across a wide range of species, including humans, mice, rats, chickens, and other animals. These kits are essential for research into inflammation, immune responses, cell signaling, and disease biomarkers.
Key Features
- Wide Range of Interleukins:
- Quantification of IL-1, IL-2, IL-4, IL-6, IL-10, IL-12, IL-17, IL-18, IL-23, IL-33, and more across various species.
- Species Compatibility:
- Kits available for humans, mice, rats, chickens, goats, sheep, camels, monkeys, dogs, and fish.
- High Sensitivity and Specificity:
- Accurate detection of low levels of interleukins with minimal cross-reactivity.
- Comprehensive Applications:
- Suitable for serum, plasma, culture media, and other biological fluids.
- Ease of Use:
- Pre-coated plates and ready-to-use reagents simplify workflows.
Applications
- Immune Research:
- Study cytokine profiles and immune regulation in health and disease.
- Inflammation Studies:
- Investigate interleukin roles in inflammatory conditions and disorders.
- Cancer Research:
- Assess interleukin involvement in tumor microenvironments and progression.
- Autoimmune Diseases:
- Explore interleukin dysregulation in conditions like rheumatoid arthritis and multiple sclerosis.
- Therapeutic Development:
- Evaluate potential drug targets and immune-modulating therapies.
- Infectious Disease Research:
- Monitor cytokine storms and immune responses in viral and bacterial infections.
- Vaccine Development:
- Analyze interleukin levels in preclinical and clinical vaccine studies.
Available Interleukins and Species
- Humans:
- IL-1, IL-6, IL-10, IL-17, IL-18, IL-22, IL-23, IL-33, and more.
- Mice/Rats:
- IL-1beta, IL-4, IL-6, IL-10, IL-12, IL-17A, IL-27, IL-35, and more.
- Chickens:
- IL-1, IL-2, IL-6, IL-10, IL-17, and IL-18.
- Fish:
- IL-1, IL-6, IL-8, IL-10, IL-12, and IL-23.
- Other Species:
- Goats, dogs, monkeys, camels, ducks, and horses.
Kit Components
- Pre-coated microtiter plates.
- Specific interleukin standards for calibration.
- HRP-conjugated detection antibodies.
- TMB substrate solution.
- Stop solution.
- Wash buffer.
- Sample diluent.
- Instruction manual.
Assay Principle
- Sample and Standard Addition:
- Add samples or interleukin standards to the pre-coated plate.
- Binding Reaction:
- HRP-conjugated detection antibodies bind to the interleukin-antibody complex.
- Color Development:
- TMB substrate reacts with HRP, producing a blue color that changes to yellow after adding the stop solution.
- Optical Density Measurement:
- Measure OD at 450 nm to determine interleukin concentrations based on a standard curve.
Storage and Stability
- Store all components at 2–8°C.
- Avoid repeated freeze-thaw cycles for optimal reagent performance.
Advantages
- Broad Coverage:
- Detects a wide variety of interleukins for comprehensive cytokine profiling.
- High Precision:
- Delivers reproducible and reliable results across experiments.
- User-Friendly Protocol:
- Simplified workflow suitable for both novice and experienced researchers.
- Versatility:
- Compatible with multiple sample types (serum, plasma, culture media, etc.).
These Interleukin ELISA Kits are indispensable for researchers studying immune responses, cytokine profiles, and disease pathophysiology. Their precision, ease of use, and versatility make them essential tools in both basic and applied immunological research.
- Antibody Source: Rat Human Sheep Mouse Chicken Goat Fish Monkey Canine Duck Avian Camel Horse
- Interleukin Sub-type: Interleukin-1 (IL-1) Interleukin-2 (IL-2) Interleukin-3 (IL-3) Interleukin-4 (IL-4) Interleukin-5 (IL-5) Interleukin-6 (IL-6) Interleukin-7 (IL-7) Interleukin-8 (IL-8) Interleukin-9 (IL-9) Interleukin-10 (IL-10) Interleukin-11 (IL-11) Interleukin-12 (IL-12) Interleukin-13 (IL-13) Interleukin-14 (IL-14) Interleukin-15 (IL-15) Interleukin-16 (IL-16) Interleukin-17 (IL-17) Interleukin-18 (IL-18) Interleukin-19 (IL-19) Interleukin-20 (IL-20) Interleukin-21 (IL-21) Interleukin-22 (IL-22) Interleukin-23 (IL-23) Interleukin-24 (IL-24) Interleukin-25 (IL-25) Interleukin-26 (IL-26) Interleukin-27 (IL-27) Interleukin-28 (IL-28) Interleukin-29 (IL-29) Interleukin-30 (IL-20) Interleukin-31 (IL-31) Interleukin-32 (IL-32) Interleukin-33 (IL-33) Interleukin-34 (IL-34) Interleukin-35 (IL-35) Interleukin-36 (IL-36) Interleukin-37 (IL-37) Interleukin-38 (IL-38) Interleukin-39 (IL-39) Interleukin-40 (IL-40) Interleukin-1beta (IL-1beta) Interleukin 1 Alpha (IL-1a) Interleukin 1 receptor antagonist , IL-1ra Interleukin-2 receptor, IL-2R Interleukin 12 (IL-12_P40) Interleukin 17a (IL-17A) Interleukin 17F (IL-17F) Interleukin 12 (IL-12/P70) Interleukin 28B (IL-28B) Interleukin 1 Receptor (IL-1R) Interleukin 6 antibody (IL-6 Ab) soluble Interleukin 6 receptor (sIL-6R) Interleukin-18 Receptor Beta (IL-18R β) Interleukin-1 Receptor 8 (IL-1R8) Soluble interleukin-1 receptor II ,IL-1sRII Soluble interleukin-2 receptor ,IL-2sRα Interleukin 41 (IL-41) Interleukin 18 Receptor 1 (IL18R1) Interleukin 4 receptor (IL-4R) Interleukin-36 gamma (IL1F9) Interleukin 36 Alpha (IL-36A) Interleukin 28A (IL-28A) Interleukin 28B (IL28B) Interleukin-1 receptor-like 1 (IL-1R4/IL-1RL1) Interleukin 33 receptor (IL-33 receptor) Interleukin 2 Receptor Beta (IL-2Rβ) Interleukin 2 Receptor Gamma (IL-2Rγ) Interleukin 2 Receptor Alpha (IL-2Ra) Soluble interleukin-1 receptor Ⅰ,IL-1sRⅠ
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