Invitrogen™ One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli, 21 x 50 µL/tube

Catalog No :

CAS Number :

Brand :

Availability :

In Stock

This combination does not exist.

Specifications:

Application	Cloning
Storage Temperature	–80°C
Product Type	cells	Forms	Freeze Dried
Product Brand	Thermo Fisher Scientific™
Product Grade	Cell Culture

Overview
Resource

Invitrogen One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli is an advanced strain optimized for the high-level expression of recombinant proteins. It is particularly suited for low-copy-number, T7-promoter-based systems, offering enhanced mRNA stability and reduced proteolysis.

Key Features:

High Protein Expression:
- Enhanced mRNA stability due to the rne131 mutation, which reduces endogenous RNase activity.
- Supports T7 RNA polymerase-driven expression, yielding high levels of recombinant protein.
Reduced Protein Degradation:
- Protease Deficiency: Lacks lon and OmpT proteases, minimizing degradation of heterologous proteins.
Optimized for Low-Copy Plasmids:
- Specifically designed for low-copy-number T7-promoter-based plasmids such as Champion™ pET vectors.
Single-Tube Transformation:
- Supplied in the One Shot™ format, allowing transformation and plating in the same tube to save time and prevent contamination.
Fast Growth and High Density:
- Exhibits rapid growth in minimal media and reaches high cell density in glucose-rich media with low acetate production.
Inducible Expression System:
- Expression of the T7 RNA polymerase is controlled by the lacUV5 promoter and induced with IPTG.

Applications:

Recombinant Protein Production:
- High-yield production of non-toxic proteins for research or industrial purposes.
T7 Promoter Systems:
- Supports low-copy-number T7 plasmids like Champion™ pET vectors.
RNA Stability Studies:
- Increased stability of mRNA for higher yields of recombinant protein.

Specifications:

Attribute	Details
Product Type	Chemically Competent Cells
Transformation Efficiency	Medium Efficiency (1 × 10⁸ cfu/μg pUC19 DNA)
Promoter	T7
RNA Stability	Enhanced (rne131 mutation)
Protein Stability	Enhanced (lon, ompT protease deficiencies)
High-throughput Compatibility	Low
Phage Resistance	Not resistant to T1 phage
Blue/White Screening	Not supported
Cloning Methylated DNA	Not supported
Format	Tube
Species	E. coli (B strain)
Plasmid Size	Suitable for low-copy-number plasmids
Storage Temperature	Cells: -80°C pUC19 DNA: -20°C S.O.C.: 4°C
Shipping Conditions	Dry Ice

Genotype:

F– ompT hsdSB (rB–, mB–) gal dcm rne131 (DE3)

Kit Contents:

Component	Quantity	Storage Conditions
BL21 Star™ (DE3) Cells	21 x 50 μL	–80°C
pUC19 DNA	50 μL (10 pg/μL)	–20°C
S.O.C. Medium	6 mL	4°C or room temperature

Advantages:

Feature	Benefit
Enhanced mRNA Stability	Increased transcript stability improves yields.
Reduced Protease Activity	Minimizes degradation of recombinant proteins.
Optimized for T7 Systems	Reliable performance with low-copy plasmids.
Convenient Format	Single-tube workflow saves time and reduces contamination risk.