0
Invitrogen™ Human IFN gamma ELISA Kit, High Sensitivity, 96 Tests
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Human IFN Gamma ELISA Kit, High Sensitivity (BMS228-2HS) is a high-performance, ultra-sensitive sandwich ELISA assay designed for quantifying IFN gamma (Interferon gamma) in human serum, plasma, and cell culture supernatants. With an analytical sensitivity of 0.06 pg/mL and a broad dynamic range (0.16–10.0 pg/mL), this kit ensures precise, reproducible detection of IFN gamma in immune and inflammatory research.
Featuring a pre-coated microwell plate and an amplification step, this high-sensitivity ELISA kit enables low-abundance cytokine quantification with minimal sample volume (50 µL). The kit is optimized for research in immunology, infectious diseases, oncology, and cytokine profiling.
Key Features & Benefits
1. Ultra-Sensitive IFN Gamma Quantification
- Analytical sensitivity: 0.06 pg/mL, enabling detection of low-abundance IFN gamma.
- Assay range: 0.16–10.0 pg/mL, ensuring accurate quantification across various sample types.
2. High-Throughput & Time-Efficient Workflow
- Total assay time: 4 hours, with only 1 hour 20 minutes hands-on time.
- Amplification reagents included for enhanced signal detection.
- Compatible with automated liquid handling systems.
3. Highly Reproducible & Validated Performance
- Low interassay CV (7.1%) and intraassay CV (6.8%) for reliable data consistency.
- Pre-validated for human serum, plasma, and supernatants.
- Each lot undergoes stringent quality control testing for sensitivity, specificity, and lot-to-lot consistency.
4. Robust & Specific Sandwich ELISA Format
- Pre-coated microplate with capture antibody ensures high target specificity.
- Biotinylated detection antibody & Streptavidin-HRP provide strong signal amplification.
- Colorimetric detection via HRP-TMB substrate, optimized for microplate readers.
5. Complete Kit with Ready-to-Use Reagents
- Includes all necessary components for streamlined workflow:
- Pre-coated 96-well microplate
- Biotinylated detection antibody
- Streptavidin-HRP & Amplification Reagents I & II
- Sample diluent, assay buffer, and standard
- Wash buffer, substrate solution, and stop solution
- Adhesive plate covers
Specifications
| Property | Details |
|---|---|
| Brand | Invitrogen™ |
| Product Name | Human IFN Gamma ELISA Kit, High Sensitivity |
| Catalog Number | BMS228-2HS |
| Assay Format | High Sensitivity Sandwich ELISA |
| Detection Method | Colorimetric (HRP-TMB) |
| Sensitivity | 0.06 pg/mL |
| Assay Range | 0.16 – 10.0 pg/mL |
| Sample Type | Human Serum, Plasma, Supernatant |
| Sample Volume | 50 µL |
| Time-to-Result | 4 hours |
| Hands-on Time | 1 hour 20 minutes |
| Intraassay CV | 6.8% |
| Interassay CV | 7.1% |
| Storage Conditions | 2 – 8°C |
| Shipping Conditions | Wet Ice |
| Instrument Compatibility | Colorimetric Microplate Reader |
Use Cases & Applications
1. Immunology & Inflammatory Research
- Quantifies IFN gamma in immune responses and inflammation models.
- Ideal for autoimmune disease research, including rheumatoid arthritis and lupus.
- Supports studies on T cell activation, cytokine signaling, and immune modulation.
2. Infectious Disease & Vaccine Research
- Measures IFN gamma secretion in viral, bacterial, and parasitic infections.
- Essential for studying immune response to vaccines and immunotherapies.
- Evaluates host-pathogen interactions and innate immunity.
3. Cancer & Immunotherapy Studies
- Investigates IFN gamma’s role in tumor immunity and anti-tumor responses.
- Helps assess immune checkpoint therapy effectiveness.
- Supports studies on T cell activation in tumor microenvironments.
4. Cytokine Profiling & Biomarker Discovery
- Enables multi-cytokine analysis for precision medicine.
- Facilitates biomarker discovery in chronic inflammatory conditions.
- Essential for studying cytokine interactions in cell culture supernatants.
Ordering Information
| Catalog Number | Description | Unit Size |
|---|---|---|
| BMS228-2HS | Human IFN Gamma ELISA Kit, High Sensitivity | 96 Tests |
Target Information: IFN Gamma (Interferon Gamma, Type II Interferon)
Interferon gamma (IFN gamma) is a proinflammatory cytokine primarily produced by T cells and natural killer (NK) cells in response to immune stimulation. It plays a crucial role in immune regulation, antiviral defense, and tumor suppression. IFN gamma functions through the JAK-STAT signaling pathway, mediating macrophage activation, MHC expression, and immunoglobulin secretion. This cytokine is a key biomarker for autoimmune diseases, infectious diseases, and cancer immunotherapy research.
Aliases:
- IFNγ, Interferon gamma, Type II Interferon, Immune Interferon
UniProt ID (Human): P01579
The Invitrogen™ Human IFN Gamma ELISA Kit, High Sensitivity (BMS228-2HS) provides ultra-sensitive and reliable quantification of IFN gamma in human samples. With a broad dynamic range (0.16–10.0 pg/mL), minimal sample volume requirement (50 µL), and validated high-performance detection, this ELISA kit is an essential tool for immunology, infectious disease, and oncology research.