Beckman Coulter™ SPRIselect™

The Beckman Coulter™ SPRIselect™ Reagent Kit is a high-precision, paramagnetic bead-based solution for nucleic acid size selection and cleanup in next-generation sequencing (NGS) library preparation. It is based on Solid Phase Reversible Immobilization (SPRI) technology, which enables customizable DNA fragment size selection without the need for gel extraction or centrifugation.

SPRIselect is optimized for manual workflows and full automation on Biomek platforms, offering high reproducibility, minimal hands-on time, and lot-to-lot consistency. This makes it ideal for low- to high-throughput sequencing workflows, improving library uniformity, yield, and overall sequencing efficiency.

Key Features & Benefits

1. SPRI-Based Nucleic Acid Size Selection

• Uses paramagnetic beads to efficiently bind and separate DNA fragments based on size.
• Enables precise, customizable selection of desired fragment sizes by adjusting bead-to-sample ratio.
• Ensures high recovery of DNA fragments, enhancing NGS sequencing quality.

2. Consistent & Reproducible Fragment Selection

• No bead calibration required between reagent lots, ensuring batch-to-batch consistency.
• Delivers highly reproducible size selection, reducing variability in library preparation.
• Minimizes size bias, which improves library complexity and uniformity.

3. Compatible with Manual & Automated Workflows

• Optimized for use with Biomek liquid handling systems, reducing hands-on time.
• Supports scalable processing, from single sample preparations to high-throughput sequencing.
• Simple protocol, with fewer steps compared to traditional gel-based size selection methods.

Specifications

Applications

1. Next-Generation Sequencing (NGS) Library Prep

• Ensures precise and reproducible size selection for Illumina, PacBio, and Oxford Nanopore platforms.
• Removes unwanted short or long DNA fragments, ensuring optimal library complexity.
• Improves sequencing coverage and uniformity by controlling fragment size distribution.

2. PCR Cleanup & Genomic DNA Purification

• Eliminates excess primers, nucleotides, and unwanted PCR byproducts.
• Used for PCR amplicon purification, restriction digest cleanup, and genome fragment isolation.
• Enhances purity and integrity of DNA samples for downstream applications.

3. High-Throughput & Automated Workflows

• Compatible with Biomek liquid handling platforms, reducing manual intervention.
• Suitable for low-, medium-, and high-throughput sequencing labs.
• Increases efficiency and accuracy of NGS library preparation workflows.

Workflow Overview

1. DNA Fragment Selection Using SPRIselect

1. Bind: DNA fragments bind to SPRI beads in the presence of buffer.
2. Wash: Unwanted fragments remain in the supernatant and are discarded.
3. Elute: The desired size-selected DNA is released from the beads and collected.

By adjusting the ratio of SPRIselect beads to DNA sample, users can fine-tune the fragment size range in the final library.

Advantages Over Traditional Methods

Storage & Handling

• Store at 4°C for long-term stability.
• Gently mix before use to resuspend magnetic beads.
• Keep away from strong magnetic fields until ready for DNA binding.

Summary

The Beckman Coulter™ SPRIselect™ Reagent Kit is a powerful, precise, and automated solution for DNA size selection in NGS library preparation. Using SPRI-based paramagnetic bead technology, it ensures high recovery rates, consistent size selection, and automation compatibility. Designed for manual and automated workflows, SPRIselect significantly improves DNA library quality, making it an essential tool for high-throughput sequencing labs.