Beckman Coulter™ Agencourt AMPure XP

The AMPure XP reagent is a trusted solution for DNA cleanup in various genomic applications such as Next-Generation Sequencing (NGS), qPCR/ddPCR/PCR, microarrays, and enzymatic reactions. It is built on SPRI (Solid Phase Reversible Immobilization) technology, utilizing paramagnetic beads for selective nucleic acid binding by type and size.

Key Features and Benefits

1. Wide Application Compatibility:
   • Suitable for PCR cleanup, DNA cleanup, and NGS library preparation.
   • Compatible with leading platforms such as Illumina, Oxford Nanopore Technologies, PacBio, and Thermo Scientific workflows.
2. Efficient DNA Recovery:
   • High recovery rates of 70–90% for amplicons larger than 100 bp.
   • Ensures retention of up to 34% more genetic information compared to other cleanup reagents.
3. Size Selection and Cleanup:
   • Predictable size selection for fragments based on bead-to-sample ratio.
   • Effectively removes contaminants like dNTPs, primers, primer dimers, salts, and other impurities.
4. Automation and Manual Processing:
   • Suitable for both manual workflows and automation on liquid handling systems.
   • Optimized for scalability, enabling high-throughput processing with minimal hands-on time.
5. Streamlined Workflow:
   • Simple bind, wash, and elute steps.
   • Minimal processing times for up to 96 or 384 samples.

Specifications

Performance Insights

• Predictable Size Selection: Using bead-to-sample ratios (e.g., 1.8X, 0.9X) ensures precise and consistent DNA fragment recovery.
• Efficient Workflow:
  • Manual cleanup: Hands-on time of 25–30 minutes for up to 96 samples.
  • Automated cleanup: Hands-on time as low as 5 minutes for batch sizes of 48 or 96 samples, with total automation reducing errors and increasing throughput.

Applications

1. NGS Library Preparation:
   • Cleanup and size selection of DNA fragments for Illumina, PacBio, and other NGS platforms.
2. PCR and qPCR:
   • Removal of excess primers, dNTPs, and contaminants for downstream analysis.
3. Microarrays:
   • Sample preparation for hybridization workflows.
4. ChIP and Enzymatic Reactions:
   • Purification of DNA from protein-DNA interactions or restriction digests.

Workflow

Manual Workflow:

1. Bind DNA to magnetic beads.
2. Separate beads from contaminants.
3. Wash beads with 70% ethanol.
4. Elute DNA.
5. Transfer to a new plate.

Automated Workflow:

1. Similar steps as the manual process but optimized for liquid handling systems like the Biomek i5 Genomics Workstation.
2. Hands-on time reduced to as low as 5 minutes for 96-sample batches.

Competitive Edge

1. High Efficiency: Recovers more genetic material compared to competitors, minimizing loss of valuable data.
2. Broad Compatibility: Works seamlessly across major sequencing and PCR platforms.
3. Automation Ready: Streamlines high-throughput applications with consistent and reproducible results.

Comparison of Applied Biosystems™ MagMAX™ Pure Bind Beads and Beckman Coulter™ Agencourt AMPure XP

Both MagMAX Pure Bind Beads and AMPure XP offer reliable solutions for DNA purification and PCR cleanup. The choice between them depends on specific laboratory needs:

• MagMAX Pure Bind Beads are advantageous for laboratories seeking high DNA recovery with the convenience of room temperature storage and integration with KingFisher automated systems.
• AMPure XP is suitable for applications requiring precise DNA size selection and is compatible with various genomic workflows, albeit with the necessity for cold storage.

Evaluating the specific requirements of your applications will guide the optimal selection between these two products.

The AMPure XP reagent is an essential tool for modern genomic workflows, ensuring reliable, efficient, and scalable DNA purification and cleanup.