Autobio AUTOF MS Reagent

The Autobio AUTOF MS Reagent Series is designed to support MALDI-TOF Mass Spectrometry Microbial Identification Systems, such as the Autobio AUTOF MS1000 and AUTOF MS2000, enabling rapid and accurate identification of bacteria, fungi, and mycobacteria.

These reagents are optimized to streamline the sample preparation, calibration, and matrix application processes required for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) microbial identification.

With precise formulation and consistency, the AUTOF MS reagent line ensures accurate spectral acquisition, improved reproducibility, and high-quality microbial identification at the species level.

Background

Traditional phenotypic and biochemical identification methods can take several hours or even days to produce results. The MALDI-TOF MS technology, combined with Autobio’s optimized reagents, allows the creation of unique microbial spectral fingerprints—mainly ribosomal proteins—within minutes.

This technology supports direct identification from positive blood cultures, enhancing infection management and antimicrobial stewardship in clinical microbiology laboratories.

Available Reagents and Accessories

Key Features

• Comprehensive reagent set: Covers all stages of MALDI-TOF sample processing — from lysis to ionization.
• High reproducibility: Consistent results across multiple runs and instruments.
• Efficient protein extraction: Rapid and effective lysis for both Gram-positive and Gram-negative bacteria.
• Precision calibration: Ensures reliable mass-to-charge (m/z) alignment for accurate species identification.
• Optimized CHCA matrix: Provides strong signal intensity and low background noise.
• Enhanced workflow integration: Designed for compatibility with Autobio AUTOF MS series and other MALDI-TOF instruments.
• Supports direct identification from blood cultures: Critical for rapid sepsis diagnostics.

Technical Highlights

• CHCA Matrix: High-purity α-Cyano-4-hydroxycinnamic acid for improved spectral clarity.
• Calibrator Composition: Ribonuclease, myoglobin, E. coli proteins for reproducible calibration peaks.
• Pretreatment Chemistry: Proprietary lysis agents optimized for bacterial and fungal cell wall disruption.
• Compatible Systems: AUTOF MS1000 / MS2000 / MS600, and other MALDI-TOF analyzers.
• Storage Conditions: 2–8°C; protect from direct light.
• Shelf Life: 12 months from manufacturing date.

Applications

• Clinical Microbiology: Identification of bacteria, fungi, and mycobacteria from cultures and blood samples.
• Pharmaceutical Microbiology: Rapid screening and contamination control.
• Food & Beverage Testing: Microbial identification in raw materials and production environments.
• Veterinary Microbiology: Identification of zoonotic and veterinary pathogens.
• Research Laboratories: Strain characterization and microbial taxonomy studies.

Unique Advantages

• Complete reagent solution for MALDI-TOF microbial identification workflows.
• High signal-to-noise spectral output ensures confident species-level identification.
• Localized reagent development tailored for accurate detection of regional microbial strains.
• Compatible with multiple microbial types, including bacteria, yeasts, and filamentous fungi.
• Easy integration with existing laboratory workflows and automation platforms (e.g., AutoMimo 1200).

References

1. Croxatto A., Prod'hom G., Greub G. Applications of MALDI-TOF MS in Clinical Diagnostic Microbiology. FEMS Microbiol Rev. 2012;36(2):380–407.
2. Claydon M.A. et al. The Rapid Identification of Intact Microorganisms Using Mass Spectrometry. Nature Biotechnol. 1996;14(11):1584–1586.
3. Holland R.D. et al. Rapid Identification of Whole Bacteria Based on Spectral Patterns Using MALDI-TOF. Rapid Commun Mass Spectrom. 1996;10(10):1227–1232.
4. Drancourt M. Detection of Microorganisms in Blood Specimens Using MALDI-TOF MS. Clin Microbiol Infect. 2010;16(11):1620–1625.
5. Hoyos-Mallecot Y. et al. Rapid Detection of Carbapenemase-Producing Strains from Blood Cultures Using MALDI-TOF. J Microbiol Methods. 2014;105:98–101.

The Autobio AUTOF MS Reagent Series offers high-quality, standardized solutions for every stage of the MALDI-TOF MS microbial identification workflow—from sample pretreatment and calibration to matrix ionization and target preparation.

Optimized for Autobio AUTOF MS systems, these reagents ensure rapid, reliable, and reproducible identification of a broad range of microorganisms in clinical, research, and industrial laboratories.

A trusted companion to the Autobio Autof MS1000/MS2000/MS600 platforms, the reagent series guarantees maximum accuracy, operational convenience, and analytical consistency for next-generation microbial mass spectrometry.