Applied Biosystems™ MagMAX™ Pure Bind Beads

The MagMAX Pure Bind Beads are advanced magnetic beads designed for efficient PCR cleanup and DNA purification, providing superior recovery of DNA fragments larger than 90 bp. These beads streamline workflows by removing impurities such as dNTPs, primers, primer-dimers, and salts, making them an excellent choice for Next-Generation Sequencing (NGS) and PCR applications.

Key Features and Benefits

1. High DNA Recovery:
   • Achieves >90% recovery for DNA fragments larger than 90 bp.
   • Consistent and reliable results for both GC-rich and standard PCR products.
2. Workflow Compatibility:
   • Integrates seamlessly with manual or automated workflows.
   • Optimized for use with KingFisher automated purification systems (Apex and Flex).
3. Simplified Setup:
   • One-bottle solution eliminates the need for multiple reagents.
   • Short protocol (~20 minutes) reduces hands-on time.
4. Scalability:
   • Compatible with various sample volumes, allowing flexibility for both small-scale and high-throughput applications.
5. Sustainability:
   • Shipped and stored at room temperature, reducing energy usage and cold storage requirements.
   • Streamlines operations with a grab-and-go design.
6. High-throughput Ready:
   • Process up to 60 µL of PCR sample volumes using KingFisher 96 deep-well plates.
   • BindIx and BindIt software available for protocol optimization.

Specifications

Applications

1. NGS Library Cleanup:
   • Effective in removing impurities to ensure high-quality library preparation for sequencing.
2. PCR Cleanup:
   • Eliminates unincorporated primers, dNTPs, and other contaminants, improving downstream accuracy.
3. Sequencing Workflow Optimization:
   • Enhances target enrichment when used with Dynabeads Streptavidin for Target Enrichment.
4. DNA Purification:
   • Recovers high-quality DNA suitable for sensitive downstream applications.

Workflow

1. Bind: DNA fragments bind to the magnetic beads.
2. Wash: Beads are washed to remove contaminants.
3. Elute: Purified DNA is eluted from the beads.
4. Transfer: DNA is transferred for downstream applications.

Automation Ready

• Optimized for use with KingFisher systems:
  • KingFisher Apex (Cat. No. 5400910).
  • KingFisher Flex (Cat. No. 5400610).
• Reduces hands-on time while ensuring reproducible results.

Comparison of Applied Biosystems™ MagMAX™ Pure Bind Beads and Beckman Coulter™ Agencourt AMPure XP

Both MagMAX Pure Bind Beads and AMPure XP offer reliable solutions for DNA purification and PCR cleanup. The choice between them depends on specific laboratory needs:

• MagMAX Pure Bind Beads are advantageous for laboratories seeking high DNA recovery with the convenience of room temperature storage and integration with KingFisher automated systems.
• AMPure XP is suitable for applications requiring precise DNA size selection and is compatible with various genomic workflows, albeit with the necessity for cold storage.

Evaluating the specific requirements of your applications will guide the optimal selection between these two products.

MagMAX Pure Bind Beads are a reliable and efficient choice for laboratories seeking consistent and high-quality DNA purification.