Applied Biosystems™ ExoSAP-IT™ Express PCR Product Cleanup

The ExoSAP-IT™ Express PCR Product Cleanup Reagent from Applied Biosystems™ is a recombinant, ready-to-use enzymatic reagent that provides a fast, reliable, and loss-free method for removing residual primers and unincorporated dNTPs from PCR reactions. In just 5 minutes, this one-tube system ensures that PCR products are clean and ready for Sanger sequencing, cloning, or mutation analysis—with 100% recovery of all DNA fragments.

Unlike column- or bead-based methods that require multiple steps and can result in sample loss, ExoSAP-IT™ Express offers maximum efficiency with zero transfer steps, reducing the risk of contamination and human error.

How It Works

The reagent contains:

• Modified Exonuclease I, which degrades single-stranded primers.
• Shrimp Alkaline Phosphatase (SAP), which inactivates unincorporated dNTPs.

These enzymes are:

• Activated at 37°C for 4 minutes
• Inactivated at 80°C for 1 minute

This simple protocol makes ExoSAP-IT Express the fastest PCR cleanup method available—with results ready for immediate downstream processing.

Use Cases

🔬 1. Sanger Sequencing Preparation

• Removes leftover primers and dNTPs to ensure high-quality sequencing reads without background noise.
• Ideal for high-throughput sequencing workflows in core facilities or genomics labs.

🧬 2. Cloning & Molecular Cloning

• Ensures accurate ligation by removing components that can interfere with restriction digestion or ligation enzymes.
• Maintains 100% recovery of amplicons, critical for low-yield or precious samples.

🧪 3. SNP Genotyping & Mutation Detection

• Eliminates false-positive SNP calls due to carryover primers or dNTPs.
• Maintains sample purity for allele-specific amplification.

🧫 4. High-Throughput & Automated PCR Cleanup

• Compatible with manual, semi-automated, or fully automated workflows.
• Available in formats suitable for 96-well plates, 8-strip tubes, and single tubes.

🔍 5. Gene Expression & Functional Genomics Studies

• Ensures clean templates for gene expression studies and library preparation.
• Enhances downstream assay accuracy and reproducibility.

Comparison to Traditional Cleanup Methods

Formats Available

All formats are shipped on dry ice and should be stored at –20°C.

Why You Should Choose ExoSAP-IT™ Express

The ExoSAP-IT™ Express PCR Product Cleanup Reagent is the gold standard for enzymatic PCR cleanup, offering a superior combination of speed, simplicity, and reliability. Whether you’re preparing samples for Sanger sequencing, cloning, or genotyping, this reagent enables consistent, contamination-free, and error-proof purification of your PCR products.

🔹 No columns, no beads, no losses

🔹 100% amplicon recovery

🔹 Compatible with standard PCR buffers and workflows

🔹 Fastest cleanup protocol on the market (5 minutes)

🔹 Ideal for high-throughput labs and small research teams alike

If you're looking for a trusted, time-saving, and high-performance solution for PCR product purification, ExoSAP-IT™ Express is the clear choice.