Apollo Sci. HEPES sodium salt ≥96%

HEPES sodium salt is a zwitterionic buffering agent widely used in biological and biochemical research. Its sodium salt form ensures high solubility and suitability for applications requiring a stable and physiologically relevant pH.

Key Features

• Purity: ≥96% (assay)
• pH: 10.0 ± 0.5 (1% solution)
• Water Content: <3.0% (by Karl Fischer titration)
• Chemical Formula: C₈H₁₇N₂NaO₄S
• Molecular Weight: 260.29 g/mol
• CAS Number: 75277-39-3
• MDL Number: MFCD00036463

Chemical and Physical Properties

Applications

1. Biological Buffer:
   • Widely used in cell culture systems to maintain stable pH.
   • Particularly effective in experiments requiring physiological pH control.
2. Biochemical Research:
   • Ensures optimal conditions for enzymatic reactions.
   • Useful in protein studies and other biochemical assays.
3. Electrophoresis and Molecular Biology:
   • Applied in buffer systems for electrophoresis and nucleic acid research.

Advantages

• High Solubility: Sodium salt form ensures easy dissolution in aqueous media.
• Stable pH: Maintains pH consistency during biological and chemical processes.
• Broad Applicability: Suitable for a range of research areas, including cell biology and protein research.

Safety Information

• Hazard Classification: Irritant; handle with care.
• Precautions:
  • Wear gloves, goggles, and protective clothing during handling.
  • Work in a well-ventilated area or under a fume hood.
• Disposal: Follow local regulations for chemical waste disposal.

Storage and Handling

• Storage Temperature: Ambient; keep in a dry environment to avoid moisture absorption.
• Shelf Life: Stable under recommended storage conditions.

Apollo Scientific HEPES Sodium Salt is a reliable biological buffer with high purity (≥96%) and stability. Its versatile applications make it ideal for cell culture, biochemical assays, and molecular biology experiments requiring consistent pH control.