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Thermo Scientific™ Invitrogen™ Countess™ 3 FL Automated Cell Counter
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Capacity (Metric): 2 samples per slide
Detection Method: Brightfield, Fluorescent
Memory: On Board, USB Drive
Sample Type: Immortalized cell lines & primary cells
Package Includes; Power Supply, Slide Holder, Light Cube Installation Tool, USB Drive, Wi-Fi Dongle, 1 Box Chamber Slide
Specifications:
| Application | Cell Analysis | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Countess™ 3 FL Automated Cell Counter is a powerful benchtop instrument combining brightfield and fluorescence detection, machine learning-based image analysis, and AI-powered automation for fast, accurate cell counting and viability assessment. Ideal for use in routine cell culture and advanced fluorescence workflows, the Countess 3 FL delivers reliable data in under 30 seconds with minimal hands-on time.
With compatibility for both disposable and reusable slides, three-channel optics (brightfield + 2 EVOS™ LED light cubes), and 21 CFR Part 11 software support, the Countess 3 FL is an essential tool for labs requiring high precision, fast results, and regulatory-ready performance.
Key Features
- Accurate: AI-driven image analysis algorithm reduces subjectivity and variability
- Flexible: Supports brightfield and dual fluorescence counting using EVOS™ light cubes
- Fast: Count live/dead cells, measure viability, and size cells in ~20 seconds
- User-Friendly: Large 1280 × 800 LCD touchscreen and intuitive software
- Versatile: Suitable for cell lines, primary cells, and isolated nuclei in scRNA-seq workflows
- Portable & Compact: Small footprint with USB and Wi-Fi data export options
- No Maintenance Required: No calibration, no routine servicing
Specifications
| Property | Detail |
|---|---|
| Product Type | Automated Cell Counter (Brightfield & Fluorescence) |
| Optical Channels | 3 total: Brightfield + 2 EVOS™ LED Light Cubes (user-selected) |
| Sample Throughput | 2 samples per slide |
| Measurement Range | 1 × 10⁴ to 1 × 10⁷ cells/mL (optimal: 1 × 10⁵ to 4 × 10⁶ cells/mL) |
| Detection Method | Colorimetric + Fluorescence (via light cubes) |
| Microscope Magnification | 2.5X |
| Camera Resolution | 5 MP |
| Display | LCD, 1280 × 800 px |
| Data Storage | Onboard, USB drive, Wi-Fi dongle included |
| Compatible Slides | Countess™ Disposable & Reusable Chamber Slides |
| Dimensions (L × W × H) | 9 × 9 × 5.5 in (228.6 × 228.6 × 139.7 mm) |
| Weight | 8 lb / 3.6 kg |
| Voltage / Frequency | 100–240 V, 50/60 Hz |
| Certifications | RoHS Compliant |
| Software | Countess 3/3 FL Software (21 CFR Part 11 compatible) |
Instrument Contents
- Countess™ 3 FL Automated Cell Counter
- Countess™ Disposable Slide Holder (1)
- Countess™ Reusable Slide Holder (1)
- Countess™ Cell Counting Chamber Slides (50/box)
- USB drive and Wi-Fi dongle
- Power adapter with international plug types
- Light cube removal tool
- Quick reference card
Bundled Options
| Catalog # | Package | Includes |
|---|---|---|
| A49866 | Countess 3 FL Starter Package | Instrument, 2 EVOS light cubes (GFP & Texas Red), reusable + 500 disposable slides, extended warranty |
| A49892 | Countess 3 FL + 3-Year Rapid Exchange Warranty | 1-day replacement policy |
| A49893 | Countess 3 FL + 3-Year AB Repair Center Warranty | Depot repair + optional loaner support |
| A50299 | Starter Package + 3-Year REX Warranty | As above, with bundled extended warranty |
| A50303 | Starter Package + 3-Year ABRC Warranty | Regulated lab option with extended repair coverage |
| AMQAF2001 | Countess 3 FL with 21 CFR Part 11 Software | FDA-compliant audit trail and signature support |
| A51025 | Standalone 21 CFR Part 11 Software Module | Can be added separately to any Countess 3 FL system |
Applications
- Mammalian and yeast cell counting
- Viability analysis using trypan blue or fluorescent dyes
- Fluorescence-based transfection assessment
- Single-cell RNA-seq and scATAC-seq prep (nuclei counting)
- Quality control in bioproduction and cell banking
- Cell size measurement and aggregate analysis
The Countess™ 3 FL Automated Cell Counter provides a cutting-edge solution for rapid, reproducible, and AI-enhanced brightfield and fluorescence-based cell counting. With minimal training, no maintenance, and advanced optical performance, it is an ideal platform for labs conducting routine cell culture as well as complex fluorescence and single-cell analysis workflows. Available with optional compliance software and extended warranty packages, Countess 3 FL is built for scalability, speed, and scientific confidence.