Invitrogen™ MgCl2 (1 M), 100mL

The Invitrogen™ Ambion™ 1 M Magnesium Chloride (MgCl₂) Solution is a high-purity, RNase-free reagent designed for molecular biology and RNA-based applications. Manufactured under stringent conditions to eliminate nuclease contamination, this ready-to-use solution is supplied in a 100 mL bottle and is certified RNase-free, making it ideal for RNA research, PCR, and enzymatic reactions.

Key Features & Benefits

1. Molecular Biology Grade – High Purity

• Certified RNase-free, ensuring contamination-free use in RNA-based applications.
• Manufactured in nuclease-free facilities to prevent contamination with DNases, RNases, and proteases.
• Stringent quality control testing at multiple stages ensures high purity and reliability.

2. Ready-to-Use & Cost-Effective

• Pre-prepared 1 M solution, eliminating the need for manual preparation and pH adjustments.
• Economical and suitable for large-scale molecular biology applications.

3. Essential Reagent for Molecular Biology & Enzymatic Reactions

• Magnesium chloride (MgCl₂) is a crucial cofactor in many biological reactions, particularly in nucleic acid amplification (PCR, RT-PCR), transcription, and translation assays.
• Enhances enzyme activity in processes such as Taq polymerase-dependent PCR, reverse transcription, and restriction enzyme digestion.

Specifications

Applications

1. Molecular Biology & RNA Applications

• Used in RNA isolation, storage, and processing.
• Suitable for cDNA synthesis, in vitro transcription, and translation.
• Ensures a contamination-free environment for RNA-based research.

2. PCR & RT-PCR (Reverse Transcription PCR)

• Essential cofactor for polymerases such as Taq DNA Polymerase.
• Helps optimize primer annealing and extension efficiency.
• Used in qPCR, endpoint PCR, and high-fidelity DNA amplification.

3. Enzymatic & Biochemical Assays

• Supports enzyme activity for restriction enzymes, polymerases, and ligases.
• Used in restriction digestion, cloning, and sequencing reactions.

4. Protein Purification & Structural Biology

• Magnesium ions play a role in stabilizing protein structures.
• Used in nucleic acid-protein interaction studies.

Pack Size & Catalog Number

Storage & Handling

• Store at room temperature.
• Keep container tightly closed to prevent contamination.
• Use sterile techniques when handling to maintain RNase-free conditions.

Summary

The Invitrogen™ Ambion™ 1 M Magnesium Chloride (MgCl₂) Solution is a high-purity, RNase-free reagent ideal for RNA applications, PCR, and enzymatic reactions. Pre-prepared and nuclease-free, it provides reliable performance and convenience for molecular biology research.