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Thermo Scientific™ Pierce™ FlexMix™ Calibration Solution, 10 mL
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Mass Range: 50–3000 m/z
Specifications:
| Application | Mass Spectrometry | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Calibration Standard | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Analytical grade | ||
Comprehensive Mass Spectrometer Calibration Standard (50–3000 m/z) for Orbitrap Tribrid Systems
The Thermo Scientific™ Pierce™ FlexMix™ Calibration Solution is a ready-to-use, multi-component LC-MS calibration mixture designed for superior mass accuracy, reproducibility, and precision in Orbitrap Fusion™, Orbitrap Fusion Lumos™, and Orbitrap ID-X™ Tribrid systems. Containing 16 highly pure, ionizable compounds, FlexMix enables both positive and negative ion mode mass calibration across an exceptionally broad mass range (50–3000 m/z), ensuring accurate mass assignment for high-resolution experiments.
Each component within the mixture has been rigorously characterized and validated to deliver consistent performance lot-to-lot. The solution is supplied ready for direct infusion from a syringe, helping analysts perform fast calibrations without dilution or preparation.
Key Features & Advantages
1. Universal Calibration for Positive & Negative Ion Modes
A single formulation supports both polarities—reducing workflow complexity and enabling complete calibration coverage for Orbitrap systems.
2. Extended Mass Range: 50–3000 m/z
Supports:
- Small molecule workflows
- Peptide & protein fragments
- Lipids and metabolites
- High-mass calibrations up to 3000 m/z
This broad range improves overall mass accuracy and maximizes instrument performance.
3. Highly Validated & ISO 9001 Manufactured
- Each lot includes a certificate of analysis (CofA)
- Verified for purity, stability, and mass accuracy
- Manufactured in ISO 9001 facilities for traceable quality assurance
4. Ready-to-Use and Highly Stable
- No dilution required
- PTFE high-purity bottle prevents leaching and contamination
- Shelf stable for up to 18 months at room temperature
5. Designed for Orbitrap Tribrid Platforms
Optimized for:
- Orbitrap Fusion
- Orbitrap Fusion Lumos
- Orbitrap ID-X
Compatible with Instrument Control Software 3.1 and later.
Composition of the FlexMix™ Calibration Solution
(Accurate Masses for Positive and Negative Ion Modes)
The FlexMix Calibration Solution contains 16 ionizable components carefully selected to provide stable, distinct signals across the entire mass range. The following table describes each compound's accurate mass in both positive (+) and negative (−) ion modes.
Complete Component & Accurate Mass Table
| Component | Positive Mode (m/z) | Negative Mode (m/z) |
|---|---|---|
| LC-MS grade acetic acid | — | 59.0128 (−) |
| Imidazole | 69.0447 (+) | — |
| Triethylamine | 102.1277 (+) | — |
| Trifluoroacetic acid (TFA) | — | 112.9845 (−) |
| Tetramethylpiperidine | 142.1590 (+) | — |
| Pentafluoropropionic acid | — | 163 (−) |
| Caffeine | 195 (+) | — |
| Hexamethoxyphosphazene (HMPA) | 322.0481 (+) | — |
| Perfluorohexanoic acid | — | 363 (−) |
| MRFA peptide (Met-Arg-Phe-Ala) | 524 (+) | — |
| 2,4,6-Tris(heptafluoropropyl)-1,3,5-triazine | — | 601.9779 (−) |
| Hexakis(2,2-difluoroethoxy)phosphazene | 622.0289 (+) | — |
| Hexakis(2,2,3,3-tetrafluoropropoxy)phosphazene | 922.0098 (+) | 1033.9870 (−) |
| Ultramark 1621 series (polyfluorinated oligomers) | 1022.0034 (+) | 1233.9742 (−) |
| 1121.9970 (+) | 1333.9678 (−) | |
| 1221.9906 (+) | 1433.9614 (−) | |
| 1321.9824 (+) | 1533.9550 (−) | |
| 1421.9778 (+) | 1633.9486 (−) | |
| 1521.9714 (+) | 1733.9422 (−) | |
| 1621.9650 (+) | 1833.9358 (−) | |
| 1721.9584 (+) | 1933.9294 (−) | |
| 1821.9528 (+) | 2033.9230 (−) | |
| 1921.9474 (+) | 2133.9166 (−) | |
| Hexakis(1h,1h,7h-perfluoroheptoxy)phosphazene | 2121.9331 (+) | 2233.9104 (−) |
| Hexakis(1h,1h,9h-perfluorononoxy)phosphazene | 2721.8948 (+) | 2833.8720 (−) |
Understanding the Component Mass Coverage
Low-Mass Range (<200 m/z)
-
Acetic acid, Imidazole, Triethylamine, TFA
Useful for small-molecule ionization calibration.
Mid-Mass Range (200–1000 m/z)
-
Caffeine, HMPA, MRFA peptide
Covers wide biological analyte mass region.
High-Mass Range (1000–3000 m/z)
- Ultramark 1621 series
-
Perfluorinated phosphazenes
Essential for Orbitrap Tribrid high-resolution calibration.
These masses create a continuous calibration ladder for extremely precise mass determination.
Specifications
| Parameter | Detail |
|---|---|
| Product Type | Calibration Solution |
| Quantity | 10 mL |
| Form | Liquid |
| Workflow Step | Mass Spectrometer Calibration |
| Equipment Compatibility | Orbitrap Fusion™, Lumos™, ID-X™ |
| Shipping Condition | Room temperature |
| Mass Range | 50–3000 m/z |
| Product Line | Pierce™ |
| Storage | Room temperature (stable up to 18 months) |
The Pierce™ FlexMix™ Calibration Solution is a high-purity, ready-to-use, 16-component calibration mixture providing full-range mass accuracy calibration (50–3000 m/z) for Orbitrap Tribrid systems in both positive and negative ion modes. Manufactured to ISO 9001 standards and optimized for direct infusion, FlexMix ensures superior instrument performance, reproducibility, and system suitability.