Applied Biosystems™ TaqMan™ Advanced miRNA Human Serum/Plasma 96-well Plates, fast

The Applied Biosystems TaqMan Advanced Serum/Plasma 96-Well Plates, Fast, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format. This product contains a set of 2 plates for profiling of up to 188 unique miRNAs in serum/plasma samples, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced Serum/Plasma 96-Well Plates include:

• Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability

• Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample

• Updated content—assay content updated for mirBase v.21

• Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required

• Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:

Number of plates: 2

Number of unique miRNAs (not including exogenous controls): 188

Exogenous controls: cel-miR-39-3p, ath-miR159a

Endogenous control: hsa-miR-16-5p

Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex

Recommended master mix: TaqMan Fast Advanced Master Mix

Notes

• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.

• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.