Liofilchem® Florfenicol FFC Antimicrobial Susceptibility Disc, 30 µg

Liofilchem® Florfenicol FFC Discs (30 µg) are high-quality antimicrobial susceptibility testing (AST) discs designed for use in the Kirby-Bauer disk diffusion method, primarily for veterinary microbiology applications. Each disc is impregnated with 30 micrograms of Florfenicol, a broad-spectrum phenicol antibiotic structurally related to chloramphenicol and thiamphenicol. Florfenicol is used exclusively in veterinary medicine and is effective against a wide range of Gram-positive and Gram-negative pathogens in livestock and aquaculture. These discs are manufactured under strict ISO 9001 and ISO 13485 standards to ensure precision, reproducibility, and sterility.

Key Features

• Veterinary-exclusive antibiotic: 30 µg of Florfenicol per disc for standardized susceptibility testing in animal health diagnostics.
• Broad-spectrum activity: Active against Pasteurella multocida, Actinobacillus pleuropneumoniae, Escherichia coli, Salmonella spp., Staphylococcus aureus, and Streptococcus suis.
• Reliable and consistent: Conforms to veterinary CLSI VAST (Veterinary Antimicrobial Susceptibility Testing) standards.
• Ready to use: Supplied sterile in cartridges or canisters with desiccant to maintain integrity.
• Color-coded and labeled: Easy identification in multi-drug testing setups.

Applications and Use Cases

1. Veterinary Clinical Microbiology

Used in veterinary diagnostic laboratories to:

• Determine susceptibility of bacterial isolates from:
  • Swine (e.g., Actinobacillus pleuropneumoniae, Streptococcus suis)
  • Cattle (e.g., Mannheimia haemolytica, Pasteurella multocida)
  • Poultry (e.g., Escherichia coli)
  • Fish (e.g., Aeromonas hydrophila, Flavobacterium spp.)
• Guide treatment of:
  • Respiratory infections
  • Septicemia
  • Enteric infections in livestock and aquaculture species

2. Antimicrobial Stewardship in Animal Health

• Supports rational use of Florfenicol in veterinary practice to reduce the risk of resistance development.
• Aids in treatment monitoring and epidemiological surveillance of resistance in animal populations.

3. Research and Quality Control

• Assess resistance trends in phenicol-class antimicrobials in livestock pathogens.
• Perform comparative studies with chloramphenicol and thiamphenicol.
• Evaluate Florfenicol efficacy in feed additives, injectables, and aquaculture treatments.

Use Instructions

1. Prepare Mueller-Hinton agar or species-specific media (e.g., supplemented chocolate agar for fastidious veterinary isolates).
2. Standardize bacterial inoculum to 0.5 McFarland turbidity.
3. Inoculate plates using a sterile swab to create an even lawn of bacteria.
4. Apply a Florfenicol disc (30 µg) using sterile forceps or a disc dispenser.
5. Incubate at 35 ± 2 °C for 18–24 hours (or as recommended by veterinary guidelines).
6. Measure the zone of inhibition in millimeters.
7. Interpret using current CLSI VAST or national veterinary standards, where available.

Storage and Shelf Life

• Storage Conditions: 2–8 °C (refrigerated), protected from moisture and light.
• Shelf Life: Typically 2–3 years from manufacturing date when stored in sealed packaging.

Liofilchem® Florfenicol FFC Discs (30 µg) are indispensable tools in veterinary diagnostics, enabling accurate and standardized antimicrobial susceptibility testing for a key broad-spectrum veterinary antibiotic. Their reliability, manufacturing quality, and alignment with international veterinary standards make them essential for ensuring responsible antibiotic use and effective treatment strategies in animal health and aquaculture.