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Liofilchem® Imipenem+Phenylboronic acid IMI+BO Antimicrobial Susceptibility Discs
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Specifications:
| Application | Clinical microbiology |
| Storage Temperature | -20°C |
| Product Type | Antibiotic Disc |
| Product Brand | Liofilchem |
| Product Grade | Microbiology grade |
The Liofilchem® Imipenem+Phenylboronic Acid (IMI+BO) Antimicrobial Susceptibility Discs are specialized diagnostic tools for phenotypic detection of Klebsiella pneumoniae carbapenemase (KPC) and other Class A β-lactamases. These discs combine imipenem (a carbapenem antibiotic) and phenylboronic acid (a boronic acid derivative) to detect resistance mechanisms mediated by serine carbapenemases.
Key Features
- Selective Detection of KPC Activity:
- Phenylboronic acid inhibits KPC and other Class A carbapenemases, enhancing imipenem activity.
- Dual-Disc Synergy Method:
- Compares zones of inhibition between imipenem-only and imipenem+boronic acid discs to identify KPC-mediated resistance.
- Global Standards Compliance:
- Designed to meet CLSI and EUCAST guidelines for antimicrobial susceptibility testing.
- Broad Pathogen Application:
- Effective for Enterobacterales and non-fermenting Gram-negative bacteria.
- Ease of Use:
- Compatible with routine laboratory workflows using Mueller-Hinton Agar.
Applications
| Pathogen Group | Target Species | Mechanism Detected |
|---|---|---|
| Enterobacterales | Klebsiella pneumoniae, Escherichia coli | KPC and Class A β-lactamases |
| Non-Fermenters | Pseudomonas aeruginosa | Differentiation from efflux pumps |
| Others | Serratia spp., Proteus mirabilis | Serine β-lactamase activity |
Technical Specifications
| Feature | Details |
|---|---|
| Active Ingredients | Imipenem (10 µg), Phenylboronic Acid (400 µg) |
| Disc Diameter | 6 mm |
| Recommended Media | Mueller-Hinton Agar |
| Incubation Conditions | 35–37°C for 16–20 hours |
| Shelf Life | Refer to package labeling |
| Storage Conditions | -20°C for long-term; 2–8°C for short-term |
| Zone Interpretation | Measured in mm, based on CLSI/EUCAST standards |
Testing Procedure
Preparation
- Prepare a bacterial suspension equivalent to 0.5 McFarland Standard.
- Evenly inoculate the suspension onto a Mueller-Hinton Agar plate.
Disc Placement
- Place the IMI+BO disc alongside an imipenem-only disc (control) with a minimum distance of 25 mm between discs.
Incubation
- Incubate the plates at 35–37°C for 16–20 hours.
Result Interpretation
- Measure the zones of inhibition around both discs and compare.
Result Interpretation
| Observation | Interpretation |
|---|---|
| Zone for IMI+BO > IMI alone by ≥5 mm | Positive for KPC or Class A β-lactamase |
| Zone diameters nearly identical for both discs | Non-KPC carbapenem resistance (e.g., porin loss) |
| Normal zone diameter for both discs | No carbapenem resistance |
Quality Control Data
| Control Strain | Expected Zone Diameter |
|---|---|
| Escherichia coli ATCC® 25922 | Susceptible, no synergy observed |
| Klebsiella pneumoniae (KPC-positive) | Zone for IMI+BO significantly larger |
| Pseudomonas aeruginosa ATCC® 27853 | No zone enhancement |
Advantages
| Feature | Benefit |
|---|---|
| High Specificity | Accurate identification of KPC-producing strains. |
| Cost-Effective | Economical phenotypic detection method. |
| Time-Efficient | Results available within 16–20 hours. |
| Broad Applicability | Effective for Enterobacterales and non-fermenters. |
Comparison with Other Detection Methods
| Parameter | IMI+BO Discs | Molecular Methods |
|---|---|---|
| Cost | Affordable | Expensive |
| Time to Results | 16–20 hours | 24–48 hours |
| Ease of Use | Simple disc diffusion method | Requires specialized equipment |
| Scope | KPC detection only | Comprehensive resistance profiling |
Reference Data
- CLSI M100 Standards: Guidelines for antimicrobial susceptibility testing.
- EUCAST Recommendations: Standards for detecting KPC-producing strains.
- Scientific Literature:
- "Boronic Acid Tests for Class A β-lactamases," Journal of Clinical Microbiology.
- "Evaluation of Phenotypic Methods for Carbapenemase Detection," International Journal of Antimicrobial Agents.
Conclusion
The Liofilchem® Imipenem+Phenylboronic Acid IMI+BO Antimicrobial Susceptibility Discs offer a reliable and cost-effective method for detecting KPC-mediated resistance in Gram-negative pathogens. With their high specificity, compatibility with standard laboratory workflows, and adherence to international guidelines, they are an essential tool for antimicrobial resistance detection and surveillance.