Liofilchem® Trimethoprim-sulfamethoxazole Antimicrobial Susceptibility Disc, SXT, 25 µg

The Liofilchem® Trimethoprim-Sulfamethoxazole Antimicrobial Susceptibility Discs are precision tools designed for the Kirby-Bauer disc diffusion method to determine bacterial susceptibility to Trimethoprim-Sulfamethoxazole (TMP-SMX). Available in two specific drug concentrations—25 µg and 2.5 µg—these discs are ideal for testing Gram-positive and Gram-negative bacterial isolates in clinical diagnostics and research.

Key Features

1. Two Drug Concentrations

• 25 µg: Standard concentration for routine susceptibility testing.
• 2.5 µg: Lower concentration for specialized testing or research applications.

2. Accurate and Reliable

• Produces well-defined zones of inhibition for clear, reproducible interpretation of results.

3. Target Pathogens

• Effective against a range of pathogens, including:
  • Gram-negative bacteria: E. coli, Klebsiella spp., Proteus spp., Salmonella spp., and Shigella spp.
  • Gram-positive bacteria: Staphylococcus aureus (including MRSA), Streptococcus pneumoniae.

4. Therapeutic Combination

• Contains Trimethoprim and Sulfamethoxazole in a 1:19 ratio, mimicking clinical dosing for optimized testing accuracy.

5. Compliance with Global Standards

• Fully compliant with EUCAST, CLSI, and ISO guidelines for antimicrobial susceptibility testing.

Applications

1. Clinical Diagnostics

• Determine bacterial susceptibility to TMP-SMX for treating:
  • Urinary tract infections (UTIs).
  • Respiratory infections.
  • Gastrointestinal infections.
  • Skin and soft tissue infections.

2. Antibiotic Resistance Monitoring

• Identify resistance trends in Gram-positive and Gram-negative pathogens in healthcare and community settings.

3. Research and Development

• Investigate TMP-SMX efficacy and resistance mechanisms in experimental studies.

4. Surveillance Studies

• Monitor the spread of TMP-SMX resistance in multidrug-resistant organisms.

Specifications

How It Works

1. Sample Preparation:
   • Prepare bacterial suspension using 0.5 McFarland standard.
2. Inoculation:
   • Spread the bacterial suspension uniformly onto a Mueller-Hinton agar plate.
3. Disc Placement:
   • Place either 25 µg or 2.5 µg Trimethoprim-Sulfamethoxazole disc on the agar surface.
4. Incubation:
   • Incubate at 35–37°C for 16–18 hours.
5. Result Interpretation:
   • Measure the diameter of the zone of inhibition and compare to interpretive standards for TMP-SMX susceptibility.

Advantages

1. Precision

• Reliable results for both routine diagnostics and specialized research.

2. Flexible Applications

• Suitable for clinical labs and research institutions, with dual concentration options.

3. Quality Assured

• Manufactured under stringent quality controls to ensure uniform drug diffusion and performance.

4. Cost-Effective

• Ideal for high-throughput laboratories with standardized workflows.

Why Choose Liofilchem® Trimethoprim-Sulfamethoxazole Discs?

• Comprehensive Testing: Two concentrations provide flexibility for routine and advanced applications.
• Accuracy and Reliability: Trusted results for clinical diagnostics and resistance monitoring.
• Regulatory Compliance: Meets international standards for antimicrobial susceptibility testing.

The Liofilchem® Trimethoprim-Sulfamethoxazole Antimicrobial Susceptibility Disc (25 µg, 2.5 µg) is a reliable and versatile tool for evaluating bacterial sensitivity, aiding in effective treatment decisions and resistance surveillance.